{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Marek Bartkuhn"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16236"],"description":["In vitro WI-38 cell model showed fibroblast inflammatory response marked by IL6 and diverse myofibroblast-to lipofibroblast differentiation upon LPS exposure"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Lysis of cells was performed on plate after removal of medium and 1x wash with PBS.","Sequencing - cDNA libraries were sequenced on the Illumina NovaSeq 6000 platform generating 50 bp paired-end reads.","Nucleic Acid Extraction - RNA RNA was extracted using the RNeasy Mini Kit automatically via QIAcube®Connect (Qiagen, Hamburg, Germany).","Library Construction - 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by cDNA sequencing library preparation utilizing the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Demutliplexing and FASTQ extraction was performed using bcl2fastq v2.20.0.422. Primary processing of FASTQ data including quality control, filtering, trimming,alignment of reads and counting of gene-sprecific genes was done using nf-core RNA-seq pipeline v3.7 Processed data files contain raw sequencing counts per gene as determined by nf-core rna-seq pipeline."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"pubmed_authors":["Ying Dong","Marek Bartkuhn"],"additional_accession":[]},"is_claimable":false,"name":"In vitro model of human derived lung fibroblasts WI-38 stimulated with LPS","description":"In vitro WI-38 cell model showed fibroblast inflammatory response marked by IL6 and diverse myofibroblast-to lipofibroblast differentiation upon LPS exposure","dates":{"release":"2026-01-08T00:00:00Z","modification":"2026-01-08T19:52:24.733Z","creation":"2025-11-26T16:57:45.923Z"},"accession":"E-MTAB-16236","cross_references":{"ENA":["ERP185688"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}