biostudies-arrayexpressMarek BartkuhnMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16236In vitro WI-38 cell model showed fibroblast inflammatory response marked by IL6 and diverse myofibroblast-to lipofibroblast differentiation upon LPS exposurebiostudies-arrayexpressSample Collection - Lysis of cells was performed on plate after removal of medium and 1x wash with PBS.Sequencing - cDNA libraries were sequenced on the Illumina NovaSeq 6000 platform generating 50 bp paired-end reads.Nucleic Acid Extraction - RNA RNA was extracted using the RNeasy Mini Kit automatically via QIAcube®Connect (Qiagen, Hamburg, Germany).Library Construction - 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by cDNA sequencing library preparation utilizing the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Demutliplexing and FASTQ extraction was performed using bcl2fastq v2.20.0.422. Primary processing of FASTQ data including quality control, filtering, trimming,alignment of reads and counting of gene-sprecific genes was done using nf-core RNA-seq pipeline v3.7 Processed data files contain raw sequencing counts per gene as determined by nf-core rna-seq pipeline.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusYing DongMarek BartkuhnfalseIn vitro model of human derived lung fibroblasts WI-38 stimulated with LPSIn vitro WI-38 cell model showed fibroblast inflammatory response marked by IL6 and diverse myofibroblast-to lipofibroblast differentiation upon LPS exposure2026-01-08T00:00:00Z2026-01-08T19:52:24.733Z2025-11-26T16:57:45.923ZE-MTAB-16236ERP185688EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184