{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Max Thorwald"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16242"],"description":["U87 cells overexpressing ApoE isoforms were treated with sulforaphane for 18 hours for comparison with a DMSO control. ApoEChimp is on the ApoE4 backbone but contains R61T which is thought to be the isoform defining residue."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Library Construction - Library preparation (mRNA library, poly A enrichment) was performed by Novogene.","Nucleic Acid Extraction - RNA purification kit (Quantabio, Extracta Plus, Cat# 95214-050) was used following the manufacturer’s protocol. RNA concentration and integrity were assessed with a Qubit 4.0 Fluorometer and Agilent Bioanalyzer prior to sequencing.","Sample Collection - 5 million cells were homogenized in TRIzol reagent using a BeadBug Benchtop Homogenizer. Brain tissue was suspended in 1 mL of TRIzol and homogenized for 6 rounds of 10 seconds homogenization and 60-second holds between each round. Following homogenization, 300 µL of chloroform was added, and samples were centrifuged with heavy gel phase-lock tubes (VWR, 10847-802) to separate the aqueous phase.","Sequencing - Library preparation (mRNA library, poly A enrichment) was performed by Novogene."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Max Thorwald"],"additional_accession":[]},"is_claimable":false,"name":"ApoE isoforms differentially alter baseline antioxidant levels critical to ferroptosis mitigation","description":"U87 cells overexpressing ApoE isoforms were treated with sulforaphane for 18 hours for comparison with a DMSO control. ApoEChimp is on the ApoE4 backbone but contains R61T which is thought to be the isoform defining residue.","dates":{"release":"2026-06-30T00:00:00Z","modification":"2026-06-30T01:00:54.216Z","creation":"2025-11-21T15:31:11.206Z"},"accession":"E-MTAB-16242","cross_references":{"ENA":["ERP185473"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}