biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMax ThorwaldIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16242U87 cells overexpressing ApoE isoforms were treated with sulforaphane for 18 hours for comparison with a DMSO control. ApoEChimp is on the ApoE4 backbone but contains R61T which is thought to be the isoform defining residue.biostudies-arrayexpressLibrary Construction - Library preparation (mRNA library, poly A enrichment) was performed by Novogene.Nucleic Acid Extraction - RNA purification kit (Quantabio, Extracta Plus, Cat# 95214-050) was used following the manufacturer’s protocol. RNA concentration and integrity were assessed with a Qubit 4.0 Fluorometer and Agilent Bioanalyzer prior to sequencing.Sample Collection - 5 million cells were homogenized in TRIzol reagent using a BeadBug Benchtop Homogenizer. Brain tissue was suspended in 1 mL of TRIzol and homogenized for 6 rounds of 10 seconds homogenization and 60-second holds between each round. Following homogenization, 300 µL of chloroform was added, and samples were centrifuged with heavy gel phase-lock tubes (VWR, 10847-802) to separate the aqueous phase.Sequencing - Library preparation (mRNA library, poly A enrichment) was performed by Novogene.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMax ThorwaldfalseApoE isoforms differentially alter baseline antioxidant levels critical to ferroptosis mitigationU87 cells overexpressing ApoE isoforms were treated with sulforaphane for 18 hours for comparison with a DMSO control. ApoEChimp is on the ApoE4 backbone but contains R61T which is thought to be the isoform defining residue.2026-06-30T00:00:00Z2026-06-30T01:00:54.216Z2025-11-21T15:31:11.206ZE-MTAB-16242ERP185473EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184