{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Xuemei Miao"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16247"],"description":["Hyperglycemia, a common transient metabolic disturbance, is strongly associated with postoperative complications, yet the underlying immunometabolic mechanisms remain poorly understood. To investigate the impact of high glucose on monocytes in Stanford type A acute aortic dissection (ATAAD), we performed RNA sequencing on CD14⁺ monocytes isolated from ATAAD patients. The isolated CD14⁺ monocytes were cultured in RPMI-1640 medium supplemented with 10% FBS and exposed to either normal glucose (5.5 mM; n=4) or high glucose (30 mM; n=4) conditions for 24 hours at 37°C with 5% CO₂. After incubation, cells were harvested for RNA extraction and subsequent RNA sequencing."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Treatment - CD14⁺ monocytes were treated with normal glucose (5.5 mM; n=4) or high glucose (30 mM; n=4) medium for 24 hours.","Sample Collection - Using magnetic beads to isolate CD14⁺ monocytes from  Stanford type-A acute aortic dissection (ATAAD) patients.CD14⁺ monocytes were cultured in RPMI-1640 medium with 10% FBS and treated with 5.5 mM or 30 mM glucose for 24 hours at 37°C, 5% CO₂.After the incubation, cells were harvested for RNA extraction and subsequent RNA sequencing.","Library Construction - After enriching eukaryotic mRNA with poly(A) tails using oligo(dT)-coupled magnetic beads, the mRNA is fragmented with a fragmentation buffer. Using this fragmented mRNA as a template and random oligonucleotides as primers, the first strand of cDNA is synthesized in an M-MuLV Reverse Transcriptase system. The RNA strand is then degraded by RNase H, and the second strand of cDNA is synthesized in a DNA Polymerase I system using dNTPs. The purified double-stranded cDNA undergoes end repair, poly(A) tailing, and sequencing adapter ligation. cDNA fragments of approximately 200 bp are selected using AMPure XP beads, followed by PCR amplification. The PCR products are purified again with AMPure XP beads to finally obtain the sequencing library.","Sequencing - The sequencing library was constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer's instructions. The prepared library was quantified using a  Qubit Fluorometer and its quality was assessed using a  Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).Sequencing was performed on an Illumina NovaSeq 6000[ platform in 150 bp paired-end mode. An average of  40 million raw reads per sample was generated. The raw image data were converted to FASTQ format using Illumina bcl2fastq2 v2.20 for downstream analysis.","Growth Protocol - CD14⁺ monocytes were cultured in RPMI-1640 medium with 10% FBS and treated with 5.5 mM or 30 mM glucose for 24 hours at 37°C, 5% CO₂.","Nucleic Acid Extraction - Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according  to the manufacturer’s protocol."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Raw sequencing reads were aligned to the human reference genome GRCh38 (or hg38) using STAR (v2.7.x) with default parameters. Gene-level quantification was performed against the GENCODE (v44) comprehensive annotation file using featureCounts (from subread v2.0.0), assigning reads to protein-coding genes. To obtain expression estimates comparable across samples, the raw counts were normalized to **Transcripts Per Million (TPM)."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Xuemei Miao"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional profile of monocytes under high glucose in Stanford A acute aortic dissection","description":"Hyperglycemia, a common transient metabolic disturbance, is strongly associated with postoperative complications, yet the underlying immunometabolic mechanisms remain poorly understood. To investigate the impact of high glucose on monocytes in Stanford type A acute aortic dissection (ATAAD), we performed RNA sequencing on CD14⁺ monocytes isolated from ATAAD patients. The isolated CD14⁺ monocytes were cultured in RPMI-1640 medium supplemented with 10% FBS and exposed to either normal glucose (5.5 mM; n=4) or high glucose (30 mM; n=4) conditions for 24 hours at 37°C with 5% CO₂. After incubation, cells were harvested for RNA extraction and subsequent RNA sequencing.","dates":{"release":"2025-12-01T00:00:00Z","modification":"2025-12-01T02:01:52.598Z","creation":"2025-11-21T13:45:53.754Z"},"accession":"E-MTAB-16247","cross_references":{"ENA":["ERP185479"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184","EFO_0003969"]}}