biostudies-arrayexpressDaewon LeeMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16248Kidneys from sated and 30-hour water-deprived adult male mice were collected for single-cell RNA sequencing to examine transcriptional responses to water deprivation. Three biological replicates per condition were pooled prior to library preparation using the 10x Genomics Chromium platform. Libraries were sequenced on an Illumina NovaSeq X Plus and analyzed with Seurat.biostudies-arrayexpressLibrary Construction - Barcoded cDNA was amplified and libraries were prepared using the 10x Genomics Chromium Single Cell 3′ Library Kit v4 following the manufacturer’s instructions.Sequencing - Libraries were sequenced on an Illumina NovaSeq X Plus platform by UCSF CAT LAB.Nucleic Acid Extraction - Single-cell suspensions were processed according to the 10x Genomics Chromium Single Cell 3′kit v4 to capture polyadenylated mRNA molecules and generate barcoded cDNA.Sample Collection - Kidneys were dissected from adult male mice. Tissues were minced and enzymatically dissociated into single-cell suspensions following standard kidney dissociation procedures.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw gene-cell count matrices were processed and normalized using Seurat (v4) in R. Data were normalized with the LogNormalize method.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq XRNA-seq of coding RNA from single cellsMus musculusDaewon LeefalseSingle-cell RNA-seq of kidneys from sated and water-deprived miceKidneys from sated and 30-hour water-deprived adult male mice were collected for single-cell RNA sequencing to examine transcriptional responses to water deprivation. Three biological replicates per condition were pooled prior to library preparation using the 10x Genomics Chromium platform. Libraries were sequenced on an Illumina NovaSeq X Plus and analyzed with Seurat.2026-09-01T00:00:00Z2026-09-01T01:00:39.11Z2025-11-21T14:15:41.192ZE-MTAB-16248ERP185480EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184