{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Marek Bartkuhn"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16258"],"description":["Pregnant wild type C57BL/6J mice were randomized at 15.5 dpc or 18.5 dpc to receive intraperitoneal (i.p.) injection of saline, LPS 50 μg/kg (LPS 50) or LPS 100 μg/kg (LPS 100). At birth, newborn pups were euthanized and 2 lobes of the right lung were removed and frozen in liquid nitrogen for RNA extraction. A total amount of 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by sequencing."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - cDNA libraries were sequenced on the Illumina NovaSeq 6000 platform generating 50 bp paired-end reads.","Sample Collection - Cranial and accessory lobes of the right lung were removed and frozen in liquid nitrogen","Nucleic Acid Extraction - RNA was extracted using the RNeasy Mini Kit automatically via QIAcube®Connect (Qiagen, Hamburg, Germany).","Library Construction - 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by cDNA sequencing library preparation utilizing the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Demutliplexing and FASTQ extraction was performed using bcl2fastq v2.20.0.422. Primary processing of FASTQ data including quality control, filtering, trimming,alignment of reads and counting of gene-sprecific genes was done using nf-core RNA-seq pipeline v3.7 Processed data files contain raw sequencing counts per gene as determined by nf-core rna-seq pipeline."],"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"pubmed_authors":["Ying Dong","Marek Bartkuhn"],"additional_accession":[]},"is_claimable":false,"name":"Prenatal LPS infection impacting on neonatal mice lung at birth","description":"Pregnant wild type C57BL/6J mice were randomized at 15.5 dpc or 18.5 dpc to receive intraperitoneal (i.p.) injection of saline, LPS 50 μg/kg (LPS 50) or LPS 100 μg/kg (LPS 100). At birth, newborn pups were euthanized and 2 lobes of the right lung were removed and frozen in liquid nitrogen for RNA extraction. A total amount of 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by sequencing.","dates":{"release":"2026-01-08T00:00:00Z","modification":"2026-01-08T19:53:11.311Z","creation":"2025-11-21T16:38:20.693Z"},"accession":"E-MTAB-16258","cross_references":{"ENA":["ERP185492"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}