biostudies-arrayexpressMarek BartkuhnMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16258Pregnant wild type C57BL/6J mice were randomized at 15.5 dpc or 18.5 dpc to receive intraperitoneal (i.p.) injection of saline, LPS 50 μg/kg (LPS 50) or LPS 100 μg/kg (LPS 100). At birth, newborn pups were euthanized and 2 lobes of the right lung were removed and frozen in liquid nitrogen for RNA extraction. A total amount of 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by sequencing.biostudies-arrayexpressSequencing - cDNA libraries were sequenced on the Illumina NovaSeq 6000 platform generating 50 bp paired-end reads.Sample Collection - Cranial and accessory lobes of the right lung were removed and frozen in liquid nitrogenNucleic Acid Extraction - RNA was extracted using the RNeasy Mini Kit automatically via QIAcube®Connect (Qiagen, Hamburg, Germany).Library Construction - 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by cDNA sequencing library preparation utilizing the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Demutliplexing and FASTQ extraction was performed using bcl2fastq v2.20.0.422. Primary processing of FASTQ data including quality control, filtering, trimming,alignment of reads and counting of gene-sprecific genes was done using nf-core RNA-seq pipeline v3.7 Processed data files contain raw sequencing counts per gene as determined by nf-core rna-seq pipeline.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAMus musculusYing DongMarek BartkuhnfalsePrenatal LPS infection impacting on neonatal mice lung at birthPregnant wild type C57BL/6J mice were randomized at 15.5 dpc or 18.5 dpc to receive intraperitoneal (i.p.) injection of saline, LPS 50 μg/kg (LPS 50) or LPS 100 μg/kg (LPS 100). At birth, newborn pups were euthanized and 2 lobes of the right lung were removed and frozen in liquid nitrogen for RNA extraction. A total amount of 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by sequencing.2026-01-08T00:00:00Z2026-01-08T19:53:11.311Z2025-11-21T16:38:20.693ZE-MTAB-16258ERP185492EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184