{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Kohei Kumegawa"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16269"],"description":["RNA-seq; glioma patient derived xenograft with upregulated M3Dq; comparison between CNO treatment and PBS"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Tumors were microdissected and flash-frozen 3–4 h after CNO (hM3Dq) or vehicle treatment in TRIzol (Thermo Fisher Scientific, MA, USA) and stored at -80 ℃ before RNA extraction.","Sequencing - The libraries were sequenced by Illumina NextSeq 550","Library Construction - Sequencing library was constructed by SMARTer strand total RNA prep kit (Takara)","Nucleic Acid Extraction - Total RNA was extracted using a RNeasy Plus kit (Qiagen)."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Aligned reads were quantified at the gene level with featureCounts (v2.0.10).","Sequence Alignment - Raw sequencing reads were trimmed to remove adaptor sequences using Skewer (v0.2.2). The processed reads were then aligned to the reference genome using STAR (v2.7.8a)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NextSeq 550"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["Kohei Kumegawa"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of glioma PDX treated with/without CNO","description":"RNA-seq; glioma patient derived xenograft with upregulated M3Dq; comparison between CNO treatment and PBS","dates":{"release":"2026-07-23T00:00:00Z","modification":"2026-07-23T04:09:52.808Z","creation":"2025-11-24T08:16:52.93Z"},"accession":"E-MTAB-16269","cross_references":{"ENA":["ERP185540"],"EFO":["EFO_0002944","EFO_0004170","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}