biostudies-arrayexpressKohei KumegawaHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16269RNA-seq; glioma patient derived xenograft with upregulated M3Dq; comparison between CNO treatment and PBSbiostudies-arrayexpressSample Collection - Tumors were microdissected and flash-frozen 3–4 h after CNO (hM3Dq) or vehicle treatment in TRIzol (Thermo Fisher Scientific, MA, USA) and stored at -80 ℃ before RNA extraction.Sequencing - The libraries were sequenced by Illumina NextSeq 550Library Construction - Sequencing library was constructed by SMARTer strand total RNA prep kit (Takara)Nucleic Acid Extraction - Total RNA was extracted using a RNeasy Plus kit (Qiagen).OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Aligned reads were quantified at the gene level with featureCounts (v2.0.10).Sequence Alignment - Raw sequencing reads were trimmed to remove adaptor sequences using Skewer (v0.2.2). The processed reads were then aligned to the reference genome using STAR (v2.7.8a).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 550RNA-seq of coding RNAHomo sapiensKohei KumegawafalseRNA-seq of glioma PDX treated with/without CNORNA-seq; glioma patient derived xenograft with upregulated M3Dq; comparison between CNO treatment and PBS2026-07-23T00:00:00Z2026-07-23T04:09:52.808Z2025-11-24T08:16:52.93ZE-MTAB-16269ERP185540EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184