biostudies-arrayexpressjiayu chenMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16271Investigating the underlying mechanisms of hepatic lipid accumulation in hyperuricemic conditions. Using hepatic uricase knockout mice as a disease model, we examined transcriptional changes occurring in the mouse liver under hyperuricemic conditions, integrating these findings with the lipid accumulation phenotype.biostudies-arrayexpressNucleic Acid Extraction - Animal samples were extracted using the Trizol method. The extracted RNA was dissolved in 50 μL of DEPC-treated water. The quantity and quality of the total RNA were assessed using a Qubit fluorometer and a Qsep400 high-throughput biofragment analyzer.Sequencing - The qualified libraries were sequenced on either an Illumina platform or a BGI sequencing platform to generate 150 bp paired-end reads. Sequencing was performed using the sequencing-by-synthesis (SBS) principle.Library Construction - Strand-specific RNA-seq libraries were prepared. Briefly, mRNA was enriched from total RNA using Oligo(dT) magnetic beads. The purified mRNA was fragmented using fragmentation buffer at an elevated temperature. First-strand cDNA was synthesized using random hexamer primers. Second-strand cDNA was synthesized incorporating dUTP in place of dTTP to achieve strand specificity, followed by end repair, A-tailing, and adapter ligation. Libraries were size-selected for insert sizes of 250-350 bp using DNA magnetic beads and then amplified by PCR. Final library quality was assessed using a Qubit fluorometer and a Qsep400 analyzer. For the DNB platform, the double-stranded DNA library was circularized to form single-stranded circular DNA, which was then rolled-circle amplified to produce DNA nanoballs (DNBs).Sample Collection - Mice were euthanized by cervical dislocation at 12 weeks of age. The liver tissue was immediately dissected, rapidly frozen in liquid nitrogen, and subsequently stored at -80°C.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - FPKM= (mapped fragments of transcript)/(Total Count of mapped fragments (Millions) * Length of transcript (kb))MetabolomicsUnknownTranscriptomicsGenomicsProteomicsnoQubit fluorometerIllumina NovaSeq 6000Qsep400RNA-seq of total RNAMus musculusActivation of mitophagy antagonizes high uric acid-induced hepatic lipid accumulationjiayu chenfalseRNA-seq of UOX-KO Mouse LiverInvestigating the underlying mechanisms of hepatic lipid accumulation in hyperuricemic conditions. Using hepatic uricase knockout mice as a disease model, we examined transcriptional changes occurring in the mouse liver under hyperuricemic conditions, integrating these findings with the lipid accumulation phenotype.2025-11-26T00:00:00Z2026-05-30T15:45:20.467Z2025-11-24T07:57:00.311ZE-MTAB-16271ERP185523EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0003816EFO_0004184