biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsVedran FrankeNextSeq 2000RNA-seq of coding RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16306Skeletal muscle fibers are large multinucleated cells that form specialized subdomains such as the neuromuscular and myotendinous junctions (MTJ), the latter connecting muscle to tendon and transmitting contractile force. While transcriptional heterogeneity among myonuclei is established, the contribution of local translation to subdomain identity has remained unclear. To address this, we developed MTJ-AAV, a viral system that enables selective genetic targeting of MTJ myonuclei. Using MTJ-specific ribosome tagging, we uncovered extensive translational regulation underlying MTJ function and remodeling during exercise. This repository contains RNAseq data from whole myofibers and MTJ.biostudies-arrayexpressLibrary Construction - Library preparation was performed at the GenomEast platform at the Institute of Genetics and Molecular and Cellular Biology using Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus (Reference Guide - PN 1000000124514). Total RNA-Seq libraries were generated from 50 ng of total RNA using Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit and IDT for Illumina RNA UD Indexes, Ligation (Illumina, San Diego, USA), according to manufacturer’s instructions. DNA library were amplified using 14 cycles of PCR. Surplus PCR primers were further removed by two successive purifications using SPRIselect beads (Beckman-Coulter, Villepinte, France).Nucleic Acid Extraction - RNA was extracted by adding 350 µl of RLT buffer (Qiagen RNeasy kit) and following the manufacturer’s protocol, including DNase treatment. Purified RNA was stored at –80°C until further use.Sequencing - Libraries were sequenced on an Illumina NextSeq 2000 sequencer as paired-end 50 base reads. Image analysis and base calling were performed using RTA version 2.7.7 and BCL Convert version 3.8.4.Sample Collection - 10 weeks old homozygous RiboTag mice were intramuscularly injected with CK8- or MTJ-AAV carrying Cre recombinase. TA muscles were harvested one month later, snap-frozen, and stored at –80°C until processing.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMinchul KimVedran FrankefalseLocal translational program at the muscle-tendon junction endows domain identity in muscle syncytia - ExerciseSkeletal muscle fibers are large multinucleated cells that form specialized subdomains such as the neuromuscular and myotendinous junctions (MTJ), the latter connecting muscle to tendon and transmitting contractile force. While transcriptional heterogeneity among myonuclei is established, the contribution of local translation to subdomain identity has remained unclear. To address this, we developed MTJ-AAV, a viral system that enables selective genetic targeting of MTJ myonuclei. Using MTJ-specific ribosome tagging, we uncovered extensive translational regulation underlying MTJ function and remodeling during exercise. This repository contains RNAseq data from whole myofibers and MTJ.2026-09-30T00:00:00Z2026-09-30T11:47:41.049Z2025-11-27T11:44:07.488ZE-MTAB-16306ERP185743EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184