biostudies-arrayexpressAmanda MarzoMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16310Our study looks at the immune signatures, localization and distribution of immune cells in tumors that are regressing due to combination immunotherapies compared to monotherapy and untreated control mice that do not reject tumors.biostudies-arrayexpressScaning - Mouse nCounter® PanCancer IO 360™ Panel and Mouse nCounter® Metabolic Pathways PanelSample Collection - Whole tumor samples were placed in 300 uL of RNA later and put on ice. Tissue was cut andminced into 1-2mm sized pieces. Next, cut tissue was lysed and homogenized using Rotor–stator homogenizer in the presence of Buffer RLT, lysis buffer and bead-milling tubes.Sample Treatment - IL-15 complexes (PBS control) were administered intratumoral. anti-PD-1 mAb (IgG Isotype) was administered intraperitoneal.Nucleic Acid Extraction - RNA was extracted using Qiagen RNeasy Kit specific for Animal Tissues.Hybridization - Hybridized oligonucleotide-labeled probes were used for quantitation of the RNA target sequence.Labeling - Used Nanostring nCounter® PanCancer IO 360™ Panel and nCounter® Metabolic Pathways PanelMIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - Reference gene normalization using a set of housekeeping genes was performed via nCounter software.UnknownTranscriptomicsGenomicsProteomicsNAnCounter Pro and nCounter Pro Analysis Systemtranscription profiling by arrayMus musculusAmanda MarzofalseNanoString analysis of mouse breast tumor (EO771) treated with IL-15 complexes and/or anti-PD-1, and PBS/IgG as controlOur study looks at the immune signatures, localization and distribution of immune cells in tumors that are regressing due to combination immunotherapies compared to monotherapy and untreated control mice that do not reject tumors.2025-12-20T00:00:00Z2025-12-20T02:02:02.067Z2025-11-28T12:31:16.246ZE-MTAB-16310EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0005518EFO_0003816EFO_0003815EFO_0003969