{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["lanmin Liu"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16311"],"description":["HNRNPA2B1 regulates the expression and alternative splicing of apoptosis related genes with esophageal squamous cell carcinoma development in KYSE150 cells"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - KYSE150 cells (CL-0638, Procell Life Science & Technology Co., Ltd., China) were cultured at 37℃ with 5% CO2 in an RPMI-1640 and Ham's F-12 (PM150110 and PM150810, Procell Life Science & Technology Co., Ltd., China) with 10% fetal bovine serum (FBS) (10091148, Gibco, China), 100 µg/mL streptomycin, 100 U/mL penicillin (SV30010, Hyclone, USA). Cells were infected by lentivirus with MOI=50. Stable cell lines were obtained by screening with 1 µg/mL puromycin and cells were harvested for RT-qPCR and Western blotting analysis","Nucleic Acid Extraction - 48-hours post-transfection, total RNA was extracted using TRIzol reagent (Ambion).","Sequencing - Illumina NovaSeq 6000 system was used for 150 nt paired-end sequencing.","Library Construction - VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme) was used to prepare strand-specific RNA-seq libraries."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Uniquely mapped reads were used to calculate read number and paired-end fragments per kilobase of exon per million fragments mapped (FPKM) for each gene.","Sequence Alignment - Raw sequencing reads containing more than 2-N bases were first discarded. Subsequently, the raw reads were trimmed of adaptors and low-quality bases using a FASTX-Toolkit (v.0.0.13; http://hannonlab.cshl.edu/fastx toolkit/)."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Homo sapiens"],"pubmed_authors":["lanmin Liu"],"additional_accession":[]},"is_claimable":false,"name":"Regulatory mechanism of HNRNPA2B1 in human esophageal squamous cell carcinoma cells","description":"HNRNPA2B1 regulates the expression and alternative splicing of apoptosis related genes with esophageal squamous cell carcinoma development in KYSE150 cells","dates":{"release":"2026-01-09T00:00:00Z","modification":"2026-05-31T01:30:20.761Z","creation":"2025-12-01T11:46:49.808Z"},"accession":"E-MTAB-16311","cross_references":{"ENA":["ERP185896"],"EFO":["EFO_0002944","EFO_0004170","EFO_0004917","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}