biostudies-arrayexpressDaniel FinkeRattus norvegicushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16326One proposed molecular mechanism of Doxorubicin-induced cardiotoxicity is the induction of double-strand breaks. Topoisomerase IIb (TopoIIb) binding is dependent on DNA damage. We hypothesized to find novel genomic targets of Doxorubicin-induced cardiotoxixity by identifying differential TopoIIb targets after Doxorubin treatment in cardiomyocytes. Therefore, neonatal rat cardiomyocytes (NRVM) were treated with Doxorubin (1µM for 3h).biostudies-arrayexpressSample Collection - Neonatal rat hearts (P1) get extracted from the neonates and the left ventricle of each animal is further processed. They get digested in a pancreatin solution at 37°C. Every 15min the supernated is removed and the cells are pelleted at 1000rpm. The reaction is stopped by addition of full culture medium. All the cells are finally collected and myocytes are selected by a percoll gradient. Cells are plated in petri dishes at 10 million cells per plate.Nucleic Acid Extraction - Neonatal rat hearts (P1) get extracted from the neonates and the left ventricle of each animal is further processed. They get digested in a pancreatin solution at 37°C. Every 15min the supernated is removed and the cells are pelleted at 1000rpm. The reaction is stopped by addition of full culture medium. All the cells are finally collected and myocytes are selected by a percoll gradient. Cells are plated in petri dishes at 10 million cells per plate.Sequencing - Libraries are prepared according to the manufacture's protocol (NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina)Library Construction - Libraries are prepared according to the manufacture's protocol (NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina)OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Libraries are prepared according to the manufacture's protocol (NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina)MetabolomicsUnknownTranscriptomicsGenomicsProteomicsIllumina HiSeq 2000RNA-seq of coding RNARattus norvegicusDaniel FinkefalseTopoIIb ChIP-seq in Doxorubicin-treated neonatal rat cardiomyocytesOne proposed molecular mechanism of Doxorubicin-induced cardiotoxicity is the induction of double-strand breaks. Topoisomerase IIb (TopoIIb) binding is dependent on DNA damage. We hypothesized to find novel genomic targets of Doxorubicin-induced cardiotoxixity by identifying differential TopoIIb targets after Doxorubin treatment in cardiomyocytes. Therefore, neonatal rat cardiomyocytes (NRVM) were treated with Doxorubin (1µM for 3h).2026-08-17T00:00:00Z2026-08-17T01:00:42.119Z2025-12-19T14:16:38.72ZE-MTAB-16326ERP186841EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184