{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Wenfei Sun"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16330"],"description":["Single-nucleus RNA-seq reveals hepatocyte heterogeneity and progenitor-like populations in Lamtor2-deficient mouse liver Single-nucleus RNA sequencing (snRNA-seq) was performed on whole livers from control mice and from two models of hepatocyte-specific Lamtor2 deletion (a chronic developmental knockout and an acute adult knockout). Unsupervised transcriptomic analysis identified multiple liver cell types and hepatocyte subpopulations, revealing that early (developmental) loss of Lamtor2 triggers the emergence of hepatocytes with a biliary/progenitor-like gene expression signature, whereas acute adult deletion leads to a distinct hepatocyte subcluster with altered metabolic gene expression. These findings highlight hepatocyte plasticity and metabolic reprogramming in Lamtor2-deficient live"],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - the standard illumina protocol was followed","Nucleic Acid Extraction - the standard 10x 3' gene expression protocol was followed","Library Construction - the standard 10x 3' gene expression protocol was followed","Sample Treatment - A R26LSL-tdTom B Lamtor2flox/flox;R26LSL-tdTom C Lamtor2flox/flox;Albumin-Cre D Lamtor2flox/flox;R26LSL-tdTom;Albumin-Cre Treatment: Groups A and B received AAV8-TBG-Cre (2 × 10^11 GC/mouse) intraperitoneally 4 weeks before harvest; Groups C and D had no AAV treatment. Number of replicates: We generated 9 snRNA-seq libraries (biological samples) from 15 mice total across four groups (A–D). Libraries per group and mice per library: A1 (1), A2 (1); B1 (2), B2 (2), B3 (1); C1 (3), C2 (3); D1 (1), D2 (1). Some libraries were prepared from pooled tissue (2–3 mice).","Sample Collection - Mice were euthanized ad libitum, anesthetized with isoflurane and sacrificed by decapitation. Livers were dissected, weighed, snap-frozen and stored in liquid nitrogen and shipped on dry ice."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Raw data were uploaded as fastq files. Normalisation was not performed in these data."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["N/A","n/a","10x chromium controller","Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA from single cells"],"species":["Mus musculus"],"pubmed_authors":["Wenfei Sun"],"additional_accession":[]},"is_claimable":false,"name":"single nucleus RNAseq of liver samples","description":"Single-nucleus RNA-seq reveals hepatocyte heterogeneity and progenitor-like populations in Lamtor2-deficient mouse liver Single-nucleus RNA sequencing (snRNA-seq) was performed on whole livers from control mice and from two models of hepatocyte-specific Lamtor2 deletion (a chronic developmental knockout and an acute adult knockout). Unsupervised transcriptomic analysis identified multiple liver cell types and hepatocyte subpopulations, revealing that early (developmental) loss of Lamtor2 triggers the emergence of hepatocytes with a biliary/progenitor-like gene expression signature, whereas acute adult deletion leads to a distinct hepatocyte subcluster with altered metabolic gene expression. These findings highlight hepatocyte plasticity and metabolic reprogramming in Lamtor2-deficient live","dates":{"release":"2026-08-13T00:00:00Z","modification":"2026-08-13T22:04:49.927Z","creation":"2025-12-02T17:09:39.602Z"},"accession":"E-MTAB-16330","cross_references":{"ENA":["ERP185980"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005684","EFO_0005518","EFO_0003816","EFO_0004184","EFO_0003969"]}}