biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsJuliana Pipoli da FonsecaIllumina NovaSeq XRNA-seq of total RNALeishmania infantumLeishmania infantumhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16364The protozoan parasite Leishmania infantum exhibits significant genetic variability among isolates, influencing disease manifestation and treatment response. Although L. infantum is classically described as the causative agent of Visceral Leishmaniasis (VL) – often associated with immune deficiency, cases of Cutaneous Leishmaniasis (CL) caused by this species in immunocompetent individuals have been reported in different countries. With the aim to gain new insight underlying this unusual change in L. infantum tropism and pathogenicity, we applied comparative genomic and transcriptomic approaches on two canine isolates (Lcan) and two human isolates associated with CL in Tunisia. Using DNA-seq analysis, we assessed aneuploidy, gene copy number variations (CNVs), and single-nucleotide polymorbiostudies-arrayexpressNucleic Acid Extraction - The extraction was made using the Macherey Nucleospin RNA plus extraction kitSequencing - The libraries were sequenced using the Illumina Novaseq and Paired end 150 basesGrowth Protocol - Leishmania promastigotes were harvested in early passage, log phaseLibrary Construction - The library preparation was made using the Illumina Stranded Total RNA Prep and custom ribodepletion probes targeting Leishmania infantum ribosomal RNA.Sample Collection - The clinical strains were isolated on NNN medium from patients by dermal scraping, and peripheral blood of infected dogs . All Leishmania isolates were typed using multilocus enzyme electrophoresis as described by Rioux and al [15]OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesAna Maria Murta SantiJuliana Pipoli da FonsecafalseGenetic introgression and transcriptomic plasticity are associated with enhanced Leishmania infantum pathogenicity causing human cutaneous leishmaniasis in TunisiaThe protozoan parasite Leishmania infantum exhibits significant genetic variability among isolates, influencing disease manifestation and treatment response. Although L. infantum is classically described as the causative agent of Visceral Leishmaniasis (VL) – often associated with immune deficiency, cases of Cutaneous Leishmaniasis (CL) caused by this species in immunocompetent individuals have been reported in different countries. With the aim to gain new insight underlying this unusual change in L. infantum tropism and pathogenicity, we applied comparative genomic and transcriptomic approaches on two canine isolates (Lcan) and two human isolates associated with CL in Tunisia. Using DNA-seq analysis, we assessed aneuploidy, gene copy number variations (CNVs), and single-nucleotide polymor2026-09-17T00:00:00Z2026-09-17T11:43:49.749Z2025-12-04T09:54:27.12ZE-MTAB-16364ERP186097EFO_0002944EFO_0004170EFO_0009653EFO_0003789EFO_0005518EFO_0004184