{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["SOURA CHAKRABORTY"],"instrument_platform":["BD FACSAria III cell sorter","Illumina NovaSeq 6000","Novogene in-house automated library preparation system"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16365"],"description":["Human primary NK cells were isolated from peripheral blood mononuclear cells (PBMCs) and cultured under two conditions: with or without cortisol (1 µM). In parallel, primary NK cells were co-cultured with K562 target cells for 6 hours in the presence or absence of cortisol (1 µM) to assess the impact of glucocorticoid exposure on NK-cell activation. Following treatment and/or co-culture, NK cells were sorted to high purity and processed for transcriptomic profiling to identify cortisol-induced transcriptional changes in resting and target-stimulated NK cells."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - RNA-seq libraries were sequenced by Novogene using an Illumina platform, generating paired-end reads. Standard Illumina sequencing chemistry and quality control procedures were applied. Raw FASTQ files were delivered after base calling, filtering, and adapter trimming performed by Novogene.","Nucleic Acid Extraction - Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit following the manufacturer’s instructions (including on-column DNase digestion when applicable). RNA quantity and purity were assessed using NanoDrop spectrophotometry. High-quality RNA (RIN ≥ 7) was used for downstream library preparation.","Sample Collection - Human PBMC-derived NK cells were cultured with or without cortisol (1 µM) and, in parallel, co-cultured with K562 target cells for 6 hours. At the end of treatment, NK cells were sorted to high purity by flow cytometry, collected directly into RNase-free tubes, and immediately processed for RNA extraction.","Library Construction - RNA-seq libraries were generated by Novogene using their standard mRNA-seq workflow. Briefly, poly-A–enriched mRNA was isolated, fragmented, and reverse-transcribed to cDNA, followed by end repair, A-tailing, adaptor ligation, and PCR amplification. Libraries were purified, quantified, and assessed for size distribution prior to sequencing."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["SOURA CHAKRABORTY"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic profiling of human primary NK cells treated with cortisol and/or co-cultured with K562 target cells","description":"Human primary NK cells were isolated from peripheral blood mononuclear cells (PBMCs) and cultured under two conditions: with or without cortisol (1 µM). In parallel, primary NK cells were co-cultured with K562 target cells for 6 hours in the presence or absence of cortisol (1 µM) to assess the impact of glucocorticoid exposure on NK-cell activation. Following treatment and/or co-culture, NK cells were sorted to high purity and processed for transcriptomic profiling to identify cortisol-induced transcriptional changes in resting and target-stimulated NK cells.","dates":{"release":"2025-12-18T00:00:00Z","modification":"2025-12-18T16:40:14.229Z","creation":"2025-12-08T22:49:58.37Z"},"accession":"E-MTAB-16365","cross_references":{"ENA":["ERP186287"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}