biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsSOURA CHAKRABORTYBD FACSAria III cell sorterIllumina NovaSeq 6000Novogene in-house automated library preparation systemRNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16365Human primary NK cells were isolated from peripheral blood mononuclear cells (PBMCs) and cultured under two conditions: with or without cortisol (1 µM). In parallel, primary NK cells were co-cultured with K562 target cells for 6 hours in the presence or absence of cortisol (1 µM) to assess the impact of glucocorticoid exposure on NK-cell activation. Following treatment and/or co-culture, NK cells were sorted to high purity and processed for transcriptomic profiling to identify cortisol-induced transcriptional changes in resting and target-stimulated NK cells.biostudies-arrayexpressSequencing - RNA-seq libraries were sequenced by Novogene using an Illumina platform, generating paired-end reads. Standard Illumina sequencing chemistry and quality control procedures were applied. Raw FASTQ files were delivered after base calling, filtering, and adapter trimming performed by Novogene.Nucleic Acid Extraction - Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit following the manufacturer’s instructions (including on-column DNase digestion when applicable). RNA quantity and purity were assessed using NanoDrop spectrophotometry. High-quality RNA (RIN ≥ 7) was used for downstream library preparation.Sample Collection - Human PBMC-derived NK cells were cultured with or without cortisol (1 µM) and, in parallel, co-cultured with K562 target cells for 6 hours. At the end of treatment, NK cells were sorted to high purity by flow cytometry, collected directly into RNase-free tubes, and immediately processed for RNA extraction.Library Construction - RNA-seq libraries were generated by Novogene using their standard mRNA-seq workflow. Briefly, poly-A–enriched mRNA was isolated, fragmented, and reverse-transcribed to cDNA, followed by end repair, A-tailing, adaptor ligation, and PCR amplification. Libraries were purified, quantified, and assessed for size distribution prior to sequencing.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSOURA CHAKRABORTYfalseTranscriptomic profiling of human primary NK cells treated with cortisol and/or co-cultured with K562 target cellsHuman primary NK cells were isolated from peripheral blood mononuclear cells (PBMCs) and cultured under two conditions: with or without cortisol (1 µM). In parallel, primary NK cells were co-cultured with K562 target cells for 6 hours in the presence or absence of cortisol (1 µM) to assess the impact of glucocorticoid exposure on NK-cell activation. Following treatment and/or co-culture, NK cells were sorted to high purity and processed for transcriptomic profiling to identify cortisol-induced transcriptional changes in resting and target-stimulated NK cells.2025-12-18T00:00:00Z2025-12-18T16:40:14.229Z2025-12-08T22:49:58.37ZE-MTAB-16365ERP186287EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184