biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsFabian GrammesNextSeq 550RNA-seq of coding RNASalmo salarSalmo salarhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16378Bucket challenge of Atlantic salmon (salmo salar) with the infectious pancreatic necrosis (IPN) virus (new isolate). The fish are classified as QQ (resistant), Qq and qq (suscepible), based on their genotype of the IPN QTL. The IPN QTL is known to provide protection against the old IPN isoform.biostudies-arrayexpressSequencing - Sequencing was conducted by NovoGeneLibrary Construction - Libraries were constructed by NovoGeneNucleic Acid Extraction - Total RNA was extracted from liver stored in RNALater using RNeasy Mini kit (Qiagen®). A rotor-stator homogenizer (TissueRuptor® II, Qiagen®) was used to disrupt and homogenize the tissue. RNA was peluted according the manual. RNA was eluted in RNase-free water previously heated at 90°C. The RNA samples were labelled and stored at -80°C until processed downstreamSample Collection - Fish from the bucket challenge were sampled at 15 and 23 days post-challenge and stored in RNALater solution for downstream RNA extraction.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesFabian GrammesfalseIPN bucket challengeBucket challenge of Atlantic salmon (salmo salar) with the infectious pancreatic necrosis (IPN) virus (new isolate). The fish are classified as QQ (resistant), Qq and qq (suscepible), based on their genotype of the IPN QTL. The IPN QTL is known to provide protection against the old IPN isoform.2025-12-24T00:00:00Z2025-12-24T02:01:29.219Z2025-12-11T12:53:52.66ZE-MTAB-16378ERP186426EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184