biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsLuis LealIllumina NovaSeq 6000RNA-seq of coding RNAMarchantia polymorphaMarchantia polymorphahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16388We used RNA-seq to obtain a time course transcriptomics response on the liverwort Marchantia polymorpha as gemmae are released from dormancy and initiate germination. Dormant gemmae were collected from gemmae cups of adult thalli and subsequently subjected to different light treatments. Time point 0 was sampled without imbibition. All other samples were placed directly in 100 µl water for imbibition, keeping them wet throughout the sampling period. Imbibition for 1 to 12 hours was performed in constant light or constant darkness at 20°C. The imbibition process was interrupted by freezing the samples in liquid nitrogen after 1, 4, 8, and 12 hours. RNA-seq libraries were sequenced on single-read mode (101 bp) on an Illumina NovaSeq 6000 platform. There are two libraries per sample.biostudies-arrayexpressLibrary Construction - Individual cDNA libraries were constructed using the TruSeq stranded mRNA library preparation kit with Poly-A selection (Illumina Inc.).Nucleic Acid Extraction - RNA was extracted using an RNeasy Plant Mini Kit (Qiagen).Sequencing - Sequencing was performed on single-read mode (101 bp) in two lanes on a Illumina NovaSeq 6000 platform.Sample Collection - Dormant gemmae were collected from gemmae cups of adult thalli. Gemmae were sampled within a 5 minutes period for each of the three biological replicates.Growth Protocol - M. polymorpha sp. ruderalis was grown on soil under neutral day photoperiod (ND; 12/12; day/night). Plants were grown under cool white fluorescent light (50–60 µmol photons m−2 s−1) at 20°C constant temperature.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesLuis LealfalseRNA-seq of Marchantia polymorpha gemmae during dormancy releaseWe used RNA-seq to obtain a time course transcriptomics response on the liverwort Marchantia polymorpha as gemmae are released from dormancy and initiate germination. Dormant gemmae were collected from gemmae cups of adult thalli and subsequently subjected to different light treatments. Time point 0 was sampled without imbibition. All other samples were placed directly in 100 µl water for imbibition, keeping them wet throughout the sampling period. Imbibition for 1 to 12 hours was performed in constant light or constant darkness at 20°C. The imbibition process was interrupted by freezing the samples in liquid nitrogen after 1, 4, 8, and 12 hours. RNA-seq libraries were sequenced on single-read mode (101 bp) on an Illumina NovaSeq 6000 platform. There are two libraries per sample.2025-12-31T00:00:00Z2025-12-31T02:02:54.918Z2025-12-12T14:54:23.508ZE-MTAB-16388ERP186504EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184