biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMasa RollerIllumina HiSeq 4000RNA-seq of total RNAMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16391This study aimed to investigate transcriptional differences between liver tumours that were chemically-induced in five strains and species of mouse and rat. Male mice (Mus musculus domesticus C3H/HeOuJ and C57BL/6J, Mus musculus castaneus CAST/EiJ, and Mus Caroli CAROLI/EiJ) and rats (Fischer F344) were treated with diethylnitrosamine (DEN) to induce liver tumours. After tumour dissection, genomic DNA and RNA were simultaneously isolated and purified for library preparation and sequencing. Additional liver tissue samples were collected and processed in parallel: untreated liver from infant mice (15-day old, P15) and juvenile rats (56-day old, P56); untreated adult liver tissue; DEN-exposed adult liver tissue; spontaneous liver tumours.biostudies-arrayexpressLibrary Construction - Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina).Sample Collection - Liver tissue and liver tumours from male mice and rats were collected and snap frozen in liquid nitrogen.Nucleic Acid Extraction - Simultaneous isolation and purification of genomic DNA and total RNA from tumours, untreated normal adult liver, and DEN-exposed liver tissue was performed using the AllPrep 96 DNA/RNA Kits (Qiagen 80311), according to the manufacturer’s instructions. Total RNA from P15/P56 liver tissues was extracted using QIAzol Lysis Reagent (Qiagen 79306), according to the manufacturer''s instructions.Sequencing - Sequencing was performed on an Illumina HiSeq4000 to obtain 150bp paired-end reads.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesSarah AitkenMartin TaylorDuncan OdomMasa RollerfalseTotal RNA-sequencing of liver tumours and non-tumour liver tissue from five strains of mice and ratThis study aimed to investigate transcriptional differences between liver tumours that were chemically-induced in five strains and species of mouse and rat. Male mice (Mus musculus domesticus C3H/HeOuJ and C57BL/6J, Mus musculus castaneus CAST/EiJ, and Mus Caroli CAROLI/EiJ) and rats (Fischer F344) were treated with diethylnitrosamine (DEN) to induce liver tumours. After tumour dissection, genomic DNA and RNA were simultaneously isolated and purified for library preparation and sequencing. Additional liver tissue samples were collected and processed in parallel: untreated liver from infant mice (15-day old, P15) and juvenile rats (56-day old, P56); untreated adult liver tissue; DEN-exposed adult liver tissue; spontaneous liver tumours.2026-05-07T00:00:00Z2026-05-07T15:16:31.511Z2025-12-15T14:14:43.266ZE-MTAB-16391ERP186572E-MTAB-14144E-MTAB-14454E-MTAB-8518EFO_0002944EFO_0004170EFO_0009653EFO_0005518EFO_0004184