{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Arne Claeys"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["RNA-seq of coding RNA"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16403"],"description":["To investigate the role of RUVBL1/2 in neuroblastoma, we performed siRNA-mediated knockdown of RUVBL1 and RUVBL2 in CLB-BAR cells. Cells were transfected with Silencer Select siRNAs (Life Technologies) targeting RUVBL1 (ID S16369 and S16370) or RUVBL2 (ID S21307 and S21309) or a non-targeting scrambled control siRNA (S103650325, Qiagen), using Lipofectamine RNAiMAX, then cultured for 72 hours before RNA extraction and sequencing."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Nucleic Acid Extraction - RNA was extracted from the cell pellets using the ReliaPrep™ RNA Miniprep Systems (Promega) and the manufacturers protocol was followed.","Sequencing - Libraries were sequenced on Illumina NovaSeq X using 2 x 150 bp paired-end sequencing by BMKGENE.","Library Construction - Library construction strategy was Poly A enrichment. (Eukaryotic Transcriptome Library)","Sample Collection - Sample collection protocol: DNAse treated total RNA samples dissolved in nuclease free water"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Arne Claeys","Jimmy Van den Eynden"],"additional_accession":[]},"is_claimable":false,"name":"siRNA-mediated knockdown of RUVBL1 and RUVBL2 in CLB-BAR neuroblastoma cells","description":"To investigate the role of RUVBL1/2 in neuroblastoma, we performed siRNA-mediated knockdown of RUVBL1 and RUVBL2 in CLB-BAR cells. Cells were transfected with Silencer Select siRNAs (Life Technologies) targeting RUVBL1 (ID S16369 and S16370) or RUVBL2 (ID S21307 and S21309) or a non-targeting scrambled control siRNA (S103650325, Qiagen), using Lipofectamine RNAiMAX, then cultured for 72 hours before RNA extraction and sequencing.","dates":{"release":"2026-01-11T00:00:00Z","modification":"2026-01-11T02:02:28.522Z","creation":"2025-12-18T01:19:45.818Z"},"accession":"E-MTAB-16403","cross_references":{"ENA":["ERP186724"],"Biostudies":["E-MTAB-13137"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}