biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsArne ClaeysIllumina NovaSeq XRNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16403To investigate the role of RUVBL1/2 in neuroblastoma, we performed siRNA-mediated knockdown of RUVBL1 and RUVBL2 in CLB-BAR cells. Cells were transfected with Silencer Select siRNAs (Life Technologies) targeting RUVBL1 (ID S16369 and S16370) or RUVBL2 (ID S21307 and S21309) or a non-targeting scrambled control siRNA (S103650325, Qiagen), using Lipofectamine RNAiMAX, then cultured for 72 hours before RNA extraction and sequencing.biostudies-arrayexpressNucleic Acid Extraction - RNA was extracted from the cell pellets using the ReliaPrep™ RNA Miniprep Systems (Promega) and the manufacturers protocol was followed.Sequencing - Libraries were sequenced on Illumina NovaSeq X using 2 x 150 bp paired-end sequencing by BMKGENE.Library Construction - Library construction strategy was Poly A enrichment. (Eukaryotic Transcriptome Library)Sample Collection - Sample collection protocol: DNAse treated total RNA samples dissolved in nuclease free waterOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesArne ClaeysJimmy Van den EyndenfalsesiRNA-mediated knockdown of RUVBL1 and RUVBL2 in CLB-BAR neuroblastoma cellsTo investigate the role of RUVBL1/2 in neuroblastoma, we performed siRNA-mediated knockdown of RUVBL1 and RUVBL2 in CLB-BAR cells. Cells were transfected with Silencer Select siRNAs (Life Technologies) targeting RUVBL1 (ID S16369 and S16370) or RUVBL2 (ID S21307 and S21309) or a non-targeting scrambled control siRNA (S103650325, Qiagen), using Lipofectamine RNAiMAX, then cultured for 72 hours before RNA extraction and sequencing.2026-01-11T00:00:00Z2026-01-11T02:02:28.522Z2025-12-18T01:19:45.818ZE-MTAB-16403ERP186724E-MTAB-13137EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184