{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Zhi-Wei Wang"],"instrument_platform":["DNBSEQ-T7"],"study_type":["RNA-seq of coding RNA"],"organism":["Vigna radiata"],"species":["Vigna radiata"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16409"],"description":["This study presents transcriptome profiles of multiple tissues (root, stem, leaf, flower, and pod) from Vigna radiata var. sublobata, a wild relative of cultivated mung bean. RNA sequencing was performed using Illumina platform to investigate tissue-specific gene expression patterns. The data will facilitate understanding of gene regulation in different plant organs and provide resources for comparative genomics with cultivated Vigna radiata.  Experimental design: Tissues were collected from mature plants grown under controlled conditions. Three biological replicates per tissue type. Libraries were prepared using poly-A selection and sequenced in paired-end mode (2x150 bp).  This submission includes raw sequencing data (FASTQ files) for all samples."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - Libraries were sequenced on Illumina NovaSeq 6000 platform in paired-end mode (2×150 bp) following manufacturer's standard protocols.","Sample Collection - Samples of different tissues were collected from mature Vigna radiata var. sublobata plants grown in experimental field. Samples were immediately frozen in liquid nitrogen and stored at -80°C until RNA extraction.","Library Construction - RNA-seq libraries were prepared using NEBNext Ultra RNA Library Prep Kit. mRNA was enriched by poly-A selection. Libraries were quantified by Qubit and size distribution was checked by Bioanalyzer.","Nucleic Acid Extraction - Total RNA was extracted using TRIzol reagent according to manufacturer's instructions. RNA quality was checked by agarose gel electrophoresis and quantified using NanoDrop spectrophotometer.","Growth Protocol - Vigna radiata var. sublobata plants were grown in experimental field under standard agricultural conditions. Plants were watered regularly and fertilized according to standard cultivation practices."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Zhi-Wei Wang"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq analysis of multiple tissues in wild mung bean (Vigna radiata var. sublobata)","description":"This study presents transcriptome profiles of multiple tissues (root, stem, leaf, flower, and pod) from Vigna radiata var. sublobata, a wild relative of cultivated mung bean. RNA sequencing was performed using Illumina platform to investigate tissue-specific gene expression patterns. The data will facilitate understanding of gene regulation in different plant organs and provide resources for comparative genomics with cultivated Vigna radiata.  Experimental design: Tissues were collected from mature plants grown under controlled conditions. Three biological replicates per tissue type. Libraries were prepared using poly-A selection and sequenced in paired-end mode (2x150 bp).  This submission includes raw sequencing data (FASTQ files) for all samples.","dates":{"release":"2026-01-13T00:00:00Z","modification":"2026-01-13T02:02:23.472Z","creation":"2025-12-18T10:12:20.6Z"},"accession":"E-MTAB-16409","cross_references":{"ENA":["ERP186757"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0005518","EFO_0003738","EFO_0004184"]}}