biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsZhi-Wei WangDNBSEQ-T7RNA-seq of coding RNAVigna radiataVigna radiatahttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16409This study presents transcriptome profiles of multiple tissues (root, stem, leaf, flower, and pod) from Vigna radiata var. sublobata, a wild relative of cultivated mung bean. RNA sequencing was performed using Illumina platform to investigate tissue-specific gene expression patterns. The data will facilitate understanding of gene regulation in different plant organs and provide resources for comparative genomics with cultivated Vigna radiata. Experimental design: Tissues were collected from mature plants grown under controlled conditions. Three biological replicates per tissue type. Libraries were prepared using poly-A selection and sequenced in paired-end mode (2x150 bp). This submission includes raw sequencing data (FASTQ files) for all samples.biostudies-arrayexpressSequencing - Libraries were sequenced on Illumina NovaSeq 6000 platform in paired-end mode (2×150 bp) following manufacturer's standard protocols.Sample Collection - Samples of different tissues were collected from mature Vigna radiata var. sublobata plants grown in experimental field. Samples were immediately frozen in liquid nitrogen and stored at -80°C until RNA extraction.Library Construction - RNA-seq libraries were prepared using NEBNext Ultra RNA Library Prep Kit. mRNA was enriched by poly-A selection. Libraries were quantified by Qubit and size distribution was checked by Bioanalyzer.Nucleic Acid Extraction - Total RNA was extracted using TRIzol reagent according to manufacturer's instructions. RNA quality was checked by agarose gel electrophoresis and quantified using NanoDrop spectrophotometer.Growth Protocol - Vigna radiata var. sublobata plants were grown in experimental field under standard agricultural conditions. Plants were watered regularly and fertilized according to standard cultivation practices.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesZhi-Wei WangfalseRNA-seq analysis of multiple tissues in wild mung bean (Vigna radiata var. sublobata)This study presents transcriptome profiles of multiple tissues (root, stem, leaf, flower, and pod) from Vigna radiata var. sublobata, a wild relative of cultivated mung bean. RNA sequencing was performed using Illumina platform to investigate tissue-specific gene expression patterns. The data will facilitate understanding of gene regulation in different plant organs and provide resources for comparative genomics with cultivated Vigna radiata. Experimental design: Tissues were collected from mature plants grown under controlled conditions. Three biological replicates per tissue type. Libraries were prepared using poly-A selection and sequenced in paired-end mode (2x150 bp). This submission includes raw sequencing data (FASTQ files) for all samples.2026-01-13T00:00:00Z2026-01-13T02:02:23.472Z2025-12-18T10:12:20.6ZE-MTAB-16409ERP186757EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184