biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsJunya Mizoitranscription profiling by arraySolanum lycopersicumSolanum lycopersicumhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16411To investigate gene expression changes in tomato under low-phosphate conditions, plants were grown hydroponically using either a standard nutrient solution containing normal phosphate levels or a modified solution in which phosphate concentration was reduced to 1% of the control. Leaves were harvested two weeks after transplantation, and total RNA was extracted for microarray-based comparison of gene expression between the two phosphate regimes.biostudies-arrayexpressSample Collection - Leaf blades were cut from the plants, and the samples were frozen in liquid nitrogen.Nucleic Acid Extraction - Total RNA was isolated from the frozen samples using RNA iso plus (TaKaRa). Three seedlings were pooled to prepare an RNA sample.Growth Protocol - Tomato seedlings were transplanted at the age of 4 w old and grown for 2 w hydroponically with different concentrations of nutrients. The phosphate concentration in the control solution (P100%) and low phosphate solution (P1%) were 100% and 1%, respectively.Hybridization - Two sets of samples (biological repeats) were analyzed using a Cy3 and Cy5 dye swap (technical repeats).Labeling - Total RNA (250 ng) was labeled with a Low RNA Input Linear Amplification/Labeling Kit (Agilent Technologies) according to the manufacturer's instructions.Scaning - Microarray slides were scanned (scanner model G2505C with scan control software, version A.8.4; Agilent Technologies) and the data were analyzed using Feature Extraction software, version 11.5.1.1 (Agilent technologies) using the default settings.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsJunya MizoiData Transformation - Data was processed using the Subio Platform software. Normalization was done by selecting LOWESS Normalization. One representative probe was selected for each locus.falseMicroarray analysis of tomato leaves grown hydroponically under low-phosphate conditionTo investigate gene expression changes in tomato under low-phosphate conditions, plants were grown hydroponically using either a standard nutrient solution containing normal phosphate levels or a modified solution in which phosphate concentration was reduced to 1% of the control. Leaves were harvested two weeks after transplantation, and total RNA was extracted for microarray-based comparison of gene expression between the two phosphate regimes.2026-01-06T00:00:00Z2026-05-27T16:14:22.784Z2025-12-18T11:30:05.064ZE-MTAB-16411E-MTAB-16410EFO_0002768EFO_0002944EFO_0003814EFO_0003813EFO_0003789EFO_0005518EFO_0003816EFO_0003815