biostudies-arrayexpressMaoxuan LinHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16412This single cell RNA-seq experiment was performed to quantify DLL3 expression in tumor cells in small cell lung cancer patients.Tumors were rapidly dissociated after the surgical procedure using the Miltenyi Biotec Human Tumor Dissociation kit (cat# 130-095-929). Libraries were constructed using the VDJ NextGEM v1.1 10x Genomics Chromium kit according to the manufacturer's instructions. Samples were sequenced on a NextSeq 550 sequencer (Illumina). Corresponding EGA study number: EGAS50000001400, EGA dataset number: EGAD50000002034.biostudies-arrayexpressSequencing - Samples were sequenced on a NextSeq 550 sequencer (Illumina).Sample Collection - Tumors were rapidly dissociated after the surgical procedure using the Miltenyi Biotec Human Tumor Dissociation kit (cat# 130-095-929). Each lesion was minced into 1-2mm fragments with surgical scissors in a separate 1.5ml Eppendorf tube containing 932µl DMEM, 42µl of enzyme H, 21µl od enzyme R, and 5µl of enzyme A. Next, tubes were briefly vortexed and placed in a thermomixer for 15 minutes at 37°C, 350rpm. Finally, the dissociated samples were washed through a 50µm filter and counted using a manual hemocytometer to determine cell viability and concentration.Library Construction - Libraries were constructed using the VDJ NextGEM v1.1 10x Genomics Chromium kit according to the manufacturer's instructions. Assessment of libraries quality was performed after cDNA and library generation, measuring the nucleic-acid concentration (Qubit dsDNA High Sensitivity kit; Invitrogen, cat# Q32854) and size distribution (High Sensitivity BioA DNA kit; Agilent, cat# 5067-4626).Nucleic Acid Extraction - Nucleic acids (RNA) were extracted from single cells within the 10x Genomics Chromium Next GEM Single Cell V(D)J v1.1 workflow.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Raw 10x Genomics single-cell RNA-seq data (BCL/FASTQ files) were processed using the Cell Ranger pipeline (10x Genomics). FASTQ files were aligned to the GRCh38 reference genome using the Cell Ranger count workflow with default parameters.UnknownTranscriptomicsGenomicsProteomicsthe VDJ NextGEM v1.1 10x Genomics Chromium kitNextSeq 550the Miltenyi Biotec Human Tumor Dissociation kit (cat# 130-095-929)RNA-seq of coding RNA from single cellsHomo sapiensMaoxuan Linfalsesingle cell RNA-seq of tumor tissues from 10 small cell lung cancer patients with or without treatmentThis single cell RNA-seq experiment was performed to quantify DLL3 expression in tumor cells in small cell lung cancer patients.Tumors were rapidly dissociated after the surgical procedure using the Miltenyi Biotec Human Tumor Dissociation kit (cat# 130-095-929). Libraries were constructed using the VDJ NextGEM v1.1 10x Genomics Chromium kit according to the manufacturer's instructions. Samples were sequenced on a NextSeq 550 sequencer (Illumina). Corresponding EGA study number: EGAS50000001400, EGA dataset number: EGAD50000002034.2025-12-11T00:00:00Z2026-05-27T15:36:25.578Z2025-12-18T16:25:39.779ZE-MTAB-16412EGAD50000002034EGAS50000001400EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0003816EFO_0004184