{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Yihang Shen"],"instrument_platform":["Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Rattus norvegicus"],"species":["Rattus norvegicus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16415"],"description":["Postpartum depression (PPD) is a prevalent neuroendocrine disorder triggered by the abrupt decline of estrogen after delivery. Estrogen withdrawal after delivery profoundly influences neurotransmission, synaptic plasticity, and neuroendocrine function within the hypothalamic pituitary gonadal (HPG) axis. Now primary hypothalamic neurons were prepared from embryonic day 18 (E18) Sprague-Dawley rats, and 1 nM 17β-estradiol (E2) treatment was performed for 24 h or 60 h to model the short and long term estrogen exposure, then additional 48 h culturing after E2 withdrawal was performed for estrogen withdrawal."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - 5 μg of RNA from different experimental groups was prepared cDNA library using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions, and sequenced on an Illumina NovaSeq 6000 platform.","Sample Collection - Primary hypothalamic neurons were prepared from embryonic day 18 (E18) Sprague-Dawley rats. Briefly, hypothalamus was rapidly dissected under sterile conditions, minced into small fragments, and digested using Pierce Primary Neuron Isolation Kit. The digestion was terminated by adding an equal volume of B-27 Neurobasel Plus Medium. Cells were gently triturated using a fire-polished Pasteur pipette, passed through a 70-µm cell strainer, and plated onto 100 µg/mL poly-D-lysine-coated culture dishes at a density of 1 × 105 cells/cm². After 4 h, the medium was replaced. Cultures were maintained at 37 °C in a humidified incubator with 5% CO₂, and half of the medium was replaced every 3 days. To keep the Kiss1 gene activity, 0.1 nM E2 was always added in medium. 1 nM (final concentration, similarly hereinafter) E2 for 24 h or 60 h to model the short and long term estrogen exposure, then for additional 48 h culturing after E2 withdrawal.","Nucleic Acid Extraction - Total RNA of 5 x 107 cells were extracted using TRIZOL (Thermo Fisher Scientific). Nanodrop 2000 (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100 (Agilent) were used to evaluate RNA concentration and quality.","Library Construction - 5 μg of RNA from different experimental groups was prepared cDNA library using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Yihang Shen"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq of primary rat hypothalamic neurons by short- and long-term estrogen stimulation and withdrawal","description":"Postpartum depression (PPD) is a prevalent neuroendocrine disorder triggered by the abrupt decline of estrogen after delivery. Estrogen withdrawal after delivery profoundly influences neurotransmission, synaptic plasticity, and neuroendocrine function within the hypothalamic pituitary gonadal (HPG) axis. Now primary hypothalamic neurons were prepared from embryonic day 18 (E18) Sprague-Dawley rats, and 1 nM 17β-estradiol (E2) treatment was performed for 24 h or 60 h to model the short and long term estrogen exposure, then additional 48 h culturing after E2 withdrawal was performed for estrogen withdrawal.","dates":{"release":"2026-01-31T00:00:00Z","modification":"2026-01-31T02:02:06.025Z","creation":"2025-12-19T18:08:43.539Z"},"accession":"E-MTAB-16415","cross_references":{"ENA":["ERP186855"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}