biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMagdalena KrocIllumina NovaSeq XChIP-seqLupinus angustifoliusLupinus angustifoliushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16430In this study we used DNA affinity purification sequencing (DAP-seq) to identify genome-wide binding of allele-specific RAP2-7 transcription factor variants in five narrow-leafed lupin (Lupinus angustifolius L.) genotypes with contrasting alkaloid regulation, including low-alkaloid iucundus line (83A:476), high-alkaloid Iucundus line (P27255) and three Bryansk low-alkaloid lines (95826 (Bryanskij-35), 95927 (Bryanskij-123) and 95928 (Bryanskij-237/83)).biostudies-arrayexpressGrowth Protocol - Plants were cultivated in a growth chamber (16 h photoperiod, 22/18 °C day/night, 55–60% relative humidity, until they reached whole plant flowering stage.Sample Collection - The immobilized Halo-tagged allele-specific RAP2-7 protein was incubated with the adapter-ligated DNA library. After incubation, the beads were washed, and the bound DNA was recovered, PCR-amplified, purified, and quantified. Negative controls were processed in parallel using adapter-ligated DNA libraries incubated with Magne HaloTag beads in the absence of recombinant protein. For more details please see accompanying manuscript.Nucleic Acid Extraction - High-molecular-weight genomic DNA for DAP-seq was extracted from frozen young leaves (3 g) using a phenol:chloroform:isoamyl alcohol protocol, and quantified fluorometrically using the Qubit dsDNA Broad Range Assay (Thermo Fisher Scientific).Library Construction - Genomic DNA libraries were prepared according to Bartlett et al. (2017; doi.org/10.1038/nprot.2017.055), with minor modifications. For more details please see accompanying manuscript.Sequencing - Samples were sequenced on an Illumina NovaSeq X Plus (PE150), at Novogene (HK) Co. Ltd., generating ~6 Gb of raw data per sample (≥20 million reads per sample).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesKatarzyna CzepielMagdalena KrocGrzegorz KoczykfalseRAP2-7 DAP-seq (Lupinus angustifolius L.)In this study we used DNA affinity purification sequencing (DAP-seq) to identify genome-wide binding of allele-specific RAP2-7 transcription factor variants in five narrow-leafed lupin (Lupinus angustifolius L.) genotypes with contrasting alkaloid regulation, including low-alkaloid iucundus line (83A:476), high-alkaloid Iucundus line (P27255) and three Bryansk low-alkaloid lines (95826 (Bryanskij-35), 95927 (Bryanskij-123) and 95928 (Bryanskij-237/83)).2026-06-30T00:00:00Z2026-06-30T01:00:54.36Z2025-12-19T12:58:40.611ZE-MTAB-16430ERP186833EFO_0002944EFO_0004170EFO_0003789EFO_0002692EFO_0005518EFO_0004184