biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsMagdalena KrocIllumina NovaSeq XRNA-seq of coding RNALupinus angustifoliusLupinus angustifoliushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16431The aim of this experiment was to compare gene expression in leaves and stems of narrow-leafed lupin (Lupinus angustifolius L.) using Illumina RNA sequencing. Total RNA was isolated from leaves and stems of five narrow-leafed lupin (Lupinus angustifolius L.) genotypes with contrasting alkaloid regulation, including an iucundus low-alkaloid line (83A:476), a bitter Iucundus line (P27255) and three Bryansk low-alkaloid lines carrying the Iucundus-type RAP2-7 allele: 95826 (Bryanskij-35), 95927 (Bryanskij-123) and 95928 (Bryanskij-237/83). Plants, in five replication per genotype, were grown under controlled conditions optimal for growth, and leaf and stem samples were collected at flowering stage.biostudies-arrayexpressGrowth Protocol - Plants were cultivated in a growth chamber (16 h photoperiod, 22/18 °C day/night, 55–60% relative humidity, until they reached whole plant flowering stage.Library Construction - cDNA libraries for Illumina RNA-seq were prepared separately for each replicate. mRNA was purified with oligo(dT) magnetic beads, fragmented, and reverse-transcribed with random hexamer primers, followed by second-strand synthesis. Libraries were prepared through end repair, A-tailing, adaptor ligation, size selection, amplification, and purification.Sample Collection - Fully expanded young leaves from plants that had reached the whole plant flowering stage were collected into sterile plastic tubes, immediately flash frozen in liquid nitrogen and stored at −80 °C until nucleic acid extraction. Immediately after leaf sampling, stem tissue was collected from the same plants by excising segments from the upper, middle and lower parts of the main stem. Stem segments were placed into sterile microcentrifuge tubes, flash frozen in liquid nitrogen and stored at −80 °C until RNA isolation.Nucleic Acid Extraction - Total RNA was isolated from 30 mg of frozen, ground organ tissue using a Maxwell RSC Plant RNA Kit (Promega, Madison, WI, USA). RNA concentration and integrity were assessed with a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany), with a minimum RNA integrity number of 8.Sequencing - Sequencing was conducted on the Illumina NovaSeq platform (PE150) at Novogene Co. Ltd.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesKatarzyna CzepielMagdalena KrocGrzegorz KoczykfalseIllumina RNA-seq of Lupinus angustifolius L. leaf and stem transcriptomesThe aim of this experiment was to compare gene expression in leaves and stems of narrow-leafed lupin (Lupinus angustifolius L.) using Illumina RNA sequencing. Total RNA was isolated from leaves and stems of five narrow-leafed lupin (Lupinus angustifolius L.) genotypes with contrasting alkaloid regulation, including an iucundus low-alkaloid line (83A:476), a bitter Iucundus line (P27255) and three Bryansk low-alkaloid lines carrying the Iucundus-type RAP2-7 allele: 95826 (Bryanskij-35), 95927 (Bryanskij-123) and 95928 (Bryanskij-237/83). Plants, in five replication per genotype, were grown under controlled conditions optimal for growth, and leaf and stem samples were collected at flowering stage.2026-06-30T00:00:00Z2026-06-30T01:00:54.363Z2025-12-19T13:22:44.989ZE-MTAB-16431ERP186836EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184