biostudies-arrayexpressxiao HanHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16434Based on public databases, transcriptome data from NPC (GSE68799) and head and neck cancer (TCGA, HNSC) patients were downloaded, and the differentially expressed RBP-MEX3A was identified through bioinformatic analysis. An MEX3A overexpression model was subsequently established in C666-1 cells. Cell proliferation was assessed using the CCK-8 assay, and apoptosis was analyzed by flow cytometry. Transcriptome sequencing was performed to systematically identify differentially expressed genes (DEGs) and alternative splicing (AS) events induced by MEX3A overexpression.biostudies-arrayexpressNucleic Acid Extraction - 48-hours post-transfection, total RNA was extracted using TRIzol reagent (Ambion).Sample Collection - Cells transfected with siRNAs with Lipofectamin as the transfection reagent. Cells were cultured at 37°C in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 100 IU/mL penicillin-streptomycin solution under 5% CO2.Sequencing - Illumina NovaSeq 6000 system was used for 150 nt paired-end sequencing.Library Construction - VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme) was used to prepare strand-specific RNA-seq libraries.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Raw sequencing reads containing more than 2-N bases were first discarded. Subsequently, the raw reads were trimmed of adaptors and low-quality bases using a FASTX-Toolkit (v.0.0.13; http://hannonlab.cshl.edu/fastx toolkit/). In addition, short reads of less than 16 nt were dropped to retain clean reads, which were subsequently aligned to the GRch38 genome by HISAT2 .Data Transformation - Uniquely mapped reads were used to calculate read number and paired-end fragments per kilobase of exon per million fragments mapped (FPKM) for each gene.UnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensxiao HanfalseMEX3A Promotes Nasopharyngeal Carcinoma Progression by Regulating Alternative Splicing of Cell Cycle-Related GenesBased on public databases, transcriptome data from NPC (GSE68799) and head and neck cancer (TCGA, HNSC) patients were downloaded, and the differentially expressed RBP-MEX3A was identified through bioinformatic analysis. An MEX3A overexpression model was subsequently established in C666-1 cells. Cell proliferation was assessed using the CCK-8 assay, and apoptosis was analyzed by flow cytometry. Transcriptome sequencing was performed to systematically identify differentially expressed genes (DEGs) and alternative splicing (AS) events induced by MEX3A overexpression.2025-01-18T00:00:00Z2026-05-30T17:10:21.061Z2025-12-19T15:15:18.314ZE-MTAB-16434ERP186840EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184