biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsYohei SatoDNBSEQ-T7RNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16435Human FOXP3+ regulatory T cells were isolated as CD4+CD25+CD127- population from PBMCs. Cells were stimulated with TCR stimulation with the presence of rhIL-2. Naive cells were CD45RA+ and memory cells were CD45RA-.biostudies-arrayexpressLibrary Construction - Library was generated with DNBSEQ Fast RNA Library Prep Set.Sample Collection - Human Tregs were isolated by cell sorting.Sequencing - Libraries were sequenced by DNBSEQ-T7.Nucleic Acid Extraction - RNA were extracted from sorted cells by RNeasy micro kit.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYohei SatofalseRNA-seq on human naive and memory regulaotry T cells before and after TCR stimulation.Human FOXP3+ regulatory T cells were isolated as CD4+CD25+CD127- population from PBMCs. Cells were stimulated with TCR stimulation with the presence of rhIL-2. Naive cells were CD45RA+ and memory cells were CD45RA-.2026-02-01T00:00:00Z2026-02-01T02:02:24.406Z2025-12-19T16:09:19.57ZE-MTAB-16435ERP186849EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184