{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Data Submission IFOM - ETS"],"instrument_platform":["NextSeq 550"],"study_type":["DNA-seq"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16485"],"description":["To exclude big genomic variations, we assessed the presence of low-frequency genomic changes using High-depth sequencing."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Cells were collected and washed with PBS 1x and DNA was extracted with the DNeasy Blood & Tissue Kit (Qiagen, 69506). The R26 EYFP locus was amplified by PCR. PCR products were loaded on 1% agarose gel and the bands were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega) according to manufacturer’s instructions.","Sequencing - Purified PCR products from mouse cell lines were sequenced with Nextseq 550.","Library Construction - The library was prepared with the Illumina DNA Prep kit.","Nucleic Acid Extraction - DNeasy Blood and Tissue Kit (Qiagen)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Data Submission IFOM - ETS","Sara Tavella","Fabrizio D'Adda Di Fagagna","Giovanni Crisafulli"],"additional_accession":[]},"is_claimable":false,"name":"Investigating genomic stability of the R26 EYFP locus in MEFs after Cas9-induced DSB","description":"To exclude big genomic variations, we assessed the presence of low-frequency genomic changes using High-depth sequencing.","dates":{"release":"2026-05-15T00:00:00Z","modification":"2026-05-15T01:00:41.436Z","creation":"2026-01-02T14:47:08.94Z"},"accession":"E-MTAB-16485","cross_references":{"ENA":["ERP187204"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}