biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsData Submission IFOM - ETSNextSeq 550DNA-seqMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16485To exclude big genomic variations, we assessed the presence of low-frequency genomic changes using High-depth sequencing.biostudies-arrayexpressSample Collection - Cells were collected and washed with PBS 1x and DNA was extracted with the DNeasy Blood & Tissue Kit (Qiagen, 69506). The R26 EYFP locus was amplified by PCR. PCR products were loaded on 1% agarose gel and the bands were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega) according to manufacturer’s instructions.Sequencing - Purified PCR products from mouse cell lines were sequenced with Nextseq 550.Library Construction - The library was prepared with the Illumina DNA Prep kit.Nucleic Acid Extraction - DNeasy Blood and Tissue Kit (Qiagen)OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesData Submission IFOM - ETSSara TavellaFabrizio D'Adda Di FagagnaGiovanni CrisafullifalseInvestigating genomic stability of the R26 EYFP locus in MEFs after Cas9-induced DSBTo exclude big genomic variations, we assessed the presence of low-frequency genomic changes using High-depth sequencing.2026-05-15T00:00:00Z2026-05-15T01:00:41.436Z2026-01-02T14:47:08.94ZE-MTAB-16485ERP187204EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184