biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsShengrui GaoDNBSEQ-T7RNA-seq of coding RNA from single cellsMus musculusMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16487Single-cell RNA sequencing was performed on tumor tissues obtained from SCC7-bearing C3H mice to investigate the effects of Fe-Shikonin treatment on the tumor immune microenvironment. Tumor samples were collected from a phosphate-buffered saline (PBS)-treated control group and a Fe-Shikonin-treated group. In the deposited dataset, files designated as “CON1” correspond to tumor samples from the PBS control group, whereas files designated as “H1” correspond to tumor samples from the Fe-Shikonin treatment group. Single-cell libraries were constructed using the DNBSEQ-TaiM 4RS instrument (MGI) and DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0 (TaiM 4) kit (Qingdao MGI Tech Co., Ltd).All libraries were sequenced on the DNBSEQ-T7RS platform (MGI) with paired-end reads.biostudies-arrayexpressSample Treatment - Tumor-bearing mice were treated with either phosphate-buffered saline (PBS) or Fe-Shikonin according to the experimental design prior to tumor collection.Sample Collection - Tumor tissues were collected from SCC7-bearing C3H mice following PBS or Fe-Shikonin treatment. Fresh tumor samples were excised and immediately processed for downstream single-cell preparation.Library Construction - Single-cell RNA libraries were constructed using the DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0 (TaiM 4) according to the manufacturer’s instructions. Briefly, single cells were partitioned into droplets, followed by reverse transcription, cDNA amplification, and library construction with sample-specific barcodes.Sequencing - Prepared single-cell RNA libraries were sequenced on the DNBSEQ-TaiM 4RS instrument (MGI, Qingdao, China) with paired-end sequencing according to the manufacturer’s protocol.Nucleic Acid Extraction - Single-cell suspensions were prepared from tumor tissues, and cellular RNA was captured during the droplet-based single-cell RNA library preparation workflow without bulk RNA extraction.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesShengrui GaofalseSingle-cell RNA sequencing reveals immune microenvironment changes induced by Fe-Shikonin treatment in SCC7 tumorsSingle-cell RNA sequencing was performed on tumor tissues obtained from SCC7-bearing C3H mice to investigate the effects of Fe-Shikonin treatment on the tumor immune microenvironment. Tumor samples were collected from a phosphate-buffered saline (PBS)-treated control group and a Fe-Shikonin-treated group. In the deposited dataset, files designated as “CON1” correspond to tumor samples from the PBS control group, whereas files designated as “H1” correspond to tumor samples from the Fe-Shikonin treatment group. Single-cell libraries were constructed using the DNBSEQ-TaiM 4RS instrument (MGI) and DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0 (TaiM 4) kit (Qingdao MGI Tech Co., Ltd).All libraries were sequenced on the DNBSEQ-T7RS platform (MGI) with paired-end reads.2026-05-30T00:00:00Z2026-05-30T01:01:22.762Z2026-01-02T14:54:40.403ZE-MTAB-16487ERP187209EFO_0002944EFO_0004170EFO_0005684EFO_0005518EFO_0004184EFO_0003969