{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"submitter":["Jiaqing Shen"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16503"],"description":["To investigate the mechanism underpinning the effect of pregnancy on psoriasis, we conducted bulk RNA sequencing on the dorsal lesional skin of imiquimod (IMQ)-induced psoriatic dermatitis mice under pregnant and non-pregnant conditions, as well as on blank controls. This analysis revealed the critical role of crosstalk between dermal fibroblasts and macrophages."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - After euthanasia via CO₂ inhalation, mice were immersed in 75% ethanol for 2 minutes. Subsequently, dorsal skin tissues were harvested and snap-frozen in liquid nitrogen.","Nucleic Acid Extraction - RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA).","Sequencing - The sequencing libraries were clustered on the Illumina NovaSeq platform using paired-end sequencing to generate high-quality reads for each sample","Library Construction - libraries were constructed using VAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version) according to the manufacturer's instructions."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","Processed Data","MAGE-TAB Files"],"data_protocol":["Data Transformation - Sequencing data underwent quality control using fastQC and adapter trimming with Trimmomatic. Clean reads were aligned to the mouse reference genome (GRCm38) using HISAT2."],"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"instrument_platform":["NanoDrop 2000 spectrophotometer","Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"species":["Mus musculus"],"pubmed_authors":["Jiaqing Shen"],"additional_accession":[]},"is_claimable":false,"name":"Bulk RNA-Seq of imiquimod-induced psoratic dermatitis mice model during pregnancy","description":"To investigate the mechanism underpinning the effect of pregnancy on psoriasis, we conducted bulk RNA sequencing on the dorsal lesional skin of imiquimod (IMQ)-induced psoriatic dermatitis mice under pregnant and non-pregnant conditions, as well as on blank controls. This analysis revealed the critical role of crosstalk between dermal fibroblasts and macrophages.","dates":{"release":"2026-09-10T00:00:00Z","modification":"2026-09-10T04:23:49.364Z","creation":"2026-01-02T15:28:58.257Z"},"accession":"E-MTAB-16503","cross_references":{"ENA":["ERP187222"],"Biostudies":["E-MTAB-16501"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003816","EFO_0003738","EFO_0004184"]}}