{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Xiushan Dong"],"instrument_platform":["Illumina MiSeq","Illumina NovaSeq 6000"],"study_type":["RNA-seq of coding RNA"],"organism":["Mus musculus"],"species":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16505"],"description":["Metagenomic sequencing of mice with different treatments: Mice were randomly divided into donor control group (Donor + MRS), constipation model group (STC + MRS), or a Lactobacillus acidophilus treated group (STC + La): A humanized mouse model was established by intragastric administration of fecal bacterial liquid from healthy donors or STC patients on alternate days, followed by continuous administration of Lactobacillus acidophilus in treatment group. Finally, the feces of each group of mice were collected, and the intestinal microbial communities of the mice were analyzed through metagenomic sequencing. 16S rRNA sequencing of mice before and after the use antibiotics: Before and after treating the mice with antibiotics, the mice's feces were collected for 16s rRNA sequencing respectively."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sequencing - 16S rRNA gene sequencing: Purified PCR amplicons were paired-end sequenced on an Illumina MiSeq platform following standard protocols.","Sequencing - Metagenomic sequencing: Metagenome libraries were then sequenced on an Illumina NovaSeq 6000 platform with PE150 at Shanghai Biotree Biotech Co., Ltd. (Shanghai, China)","Library Construction - 16S rRNA gene sequencing:The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using primers 338F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′). Metagenomic sequencing: DNA libraries were constructed using the TruSeq Nano DNA Library Preparation Kit-Set (Illumina, USA) following the manufacturer’s instructions","Sample Collection - 16S rRNA gene sequencing/Metagenomic sequencing: Place each mouse in a separate cage and collect the fresh feces excreted by the mice within 2 hours.","Nucleic Acid Extraction - 16S rRNA gene sequencing: Total genomic DNA was extracted from mice fecal samples using the QuickGene DNA tissue kit (Kurabo, Neyagawa, Japan) following the manufacturer’s protocol. Metagenomic sequencing: Genomic DNA was extracted from fecal samples using the Magnetic Bead-Based Stool DNA Extraction Kit (BioTeKe, Beijin, chain)"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Xiushan Dong"],"additional_accession":[]},"is_claimable":false,"name":"Metagenomic sequencing of mice with different treatments / 16S rRNA sequencing of mice before and after the use antibiotics","description":"Metagenomic sequencing of mice with different treatments: Mice were randomly divided into donor control group (Donor + MRS), constipation model group (STC + MRS), or a Lactobacillus acidophilus treated group (STC + La): A humanized mouse model was established by intragastric administration of fecal bacterial liquid from healthy donors or STC patients on alternate days, followed by continuous administration of Lactobacillus acidophilus in treatment group. Finally, the feces of each group of mice were collected, and the intestinal microbial communities of the mice were analyzed through metagenomic sequencing. 16S rRNA sequencing of mice before and after the use antibiotics: Before and after treating the mice with antibiotics, the mice's feces were collected for 16s rRNA sequencing respectively.","dates":{"release":"2026-03-11T00:00:00Z","modification":"2026-03-11T14:48:29.1Z","creation":"2026-01-02T15:39:43.39Z"},"accession":"E-MTAB-16505","cross_references":{"ENA":["ERP187224"],"EFO":["EFO_0002944","EFO_0004170","EFO_0005518","EFO_0003738","EFO_0004184"]}}