biostudies-arrayexpressSho SendoMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16508The purpose of this project is to characterize the myeloid derived suppressor cells (MDSCs) in the inflamed lung of interstitial lung disease SKG mice. We compared the gene expression of lung MDSCs with MDSCs from bone marrow or spleen.BD FACSAria III (BD Biosciences) was used to sort CD11b+Gr1+ cells (MDSCs) from BM, spleen and lung of interstitial lung disease model SKG mice. Total RNA was extracted from cultured cells using Maxwell RSC simplyRNA Cells Kit (Promega). Biotinylated cRNA were prepared using GeneChip 3'IVT PLUS Reagent Kit (Thermo Fisher Scientific) according to the standard manufacturer's protocol from 100 ng total RNA. Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450. GeneChips were scanned using the GeneChip Scanner 3000 7G. The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Expression Console Software (ThermoFisher Scientific) according to the default analysis settings.biostudies-arrayexpressLabeling - Biotinylated cRNA were prepared using GeneChip 3'IVT PLUS Reagent Kit (Thermo Fisher Scientific) according to the standard manufacturer's protocol from 100 ng total RNA.Nucleic Acid Extraction - Total RNA was extracted from cultured cells using Maxwell RSC simplyRNA Cells Kit (Promega).Sample Collection - BD FACSAria III (BD Biosciences) was used to sort CD11b+Gr1+ cells (MDSCs) from BM, spleen and lung of interstitial lung disease model SKG mice.Scaning - GeneChips were scanned using the GeneChip Scanner 3000 7G. The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Expression Console Software (ThermoFisher Scientific) according to the default analysis settings.Hybridization - Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array.GeneChips were washed and stained in the GeneChip Fluidics Station 450.MIAME ScoreRaw DataOrganizationAssays and DataProcessed DataMAGE-TAB FilesArray DesignsData Transformation - We obtained the processed data (.CHP files) as well as the raw data (.CEL files) from Riken Genesis.UnknownTranscriptomicsGenomicsProteomicsGeneSpringMaxwell RSC simplyRNA Cells Kit (Promega)GeneChip 3'IVT PLUS Reagent Kit (Thermo Fisher Scientific)GeneChip Scanner 3000 7G. Expression Console Software (ThermoFisher Scientific)GeneChip Mouse Genome 430 2.0 Array A-AFFY-45BD FACSAriaIII (BD Biosciences)microRNA profiling by arrayMus musculusSho SendofalseCharacterization of Lung MDSC from SKG miceThe purpose of this project is to characterize the myeloid derived suppressor cells (MDSCs) in the inflamed lung of interstitial lung disease SKG mice. We compared the gene expression of lung MDSCs with MDSCs from bone marrow or spleen.BD FACSAria III (BD Biosciences) was used to sort CD11b+Gr1+ cells (MDSCs) from BM, spleen and lung of interstitial lung disease model SKG mice. Total RNA was extracted from cultured cells using Maxwell RSC simplyRNA Cells Kit (Promega). Biotinylated cRNA were prepared using GeneChip 3'IVT PLUS Reagent Kit (Thermo Fisher Scientific) according to the standard manufacturer's protocol from 100 ng total RNA. Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the GeneChip Fluidics Station 450. GeneChips were scanned using the GeneChip Scanner 3000 7G. The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Expression Console Software (ThermoFisher Scientific) according to the default analysis settings.2026-03-31T00:00:00Z2026-03-31T01:03:46.829Z2026-01-02T15:50:08.79ZE-MTAB-16508EFO_0002944EFO_0003814EFO_0000753EFO_0003813EFO_0005518EFO_0003816EFO_0003815