biostudies-arrayexpressAndrew KjarHomo sapiensRsubread (2.18.0)https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16510Developing and benchmarking model systems to understand neurodegeneration remains an important challenge. In this work, we overexpressed mutant MAPT (P301L,V337M) in neural organoids to understand the resulting pathology.biostudies-arrayexpressSequencing - NGS sequencing was performed via paired-end 150 bp on an Illumina NovaSeq X Plus with a targeted 50 million reads per sample.Nucleic Acid Extraction - RNA samples were extracted using the QIAGEN RNeasy micro plus kit.Library Construction - Purified RNA samples were submitted to the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core for bulk sequencing. cDNA libraries were prepared with a stranded mRNA (polyA-selected) library preparation kit.Sample Collection - Day 90 organoids were collected from culture media, washed in PBS, and then homogenized in RLT buffer with BME.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Paired-end bulk RNA-seq reads were obtained as gzipped FASTQ files. Reads were aligned to the human reference genome (hg38) using an RNA-seq–specific alignment mode with paired-end information enabled (Rsubread). Aligned BAM files were collected and quantified at the gene level using the featureCounts function. Raw counts were uploaded as the processed file.UnknownTranscriptomicsGenomicsProteomicsN/AIllumina NovaSeq 6000RNA-seq of coding RNAHomo sapiensAndrew KjarfalseBulk RNA sequencing of mutant MAPT over-expression in neural organoidsDeveloping and benchmarking model systems to understand neurodegeneration remains an important challenge. In this work, we overexpressed mutant MAPT (P301L,V337M) in neural organoids to understand the resulting pathology.2026-03-31T00:00:00Z2026-03-31T01:03:46.789Z2026-01-02T16:00:28.544ZE-MTAB-16510ERP187229EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184