biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsBahman KhahaniIllumina NovaSeq XRNA-seq of coding RNABrachypodium distachyonBrachypodium distachyonhttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16515To better resolve the pronounced transcriptional changes observed around temperature transitions, we increased sampling density at the boundaries of the thermocycle.biostudies-arrayexpressGrowth Protocol - Seeds were imbibed for 21 days at 4°C and then planted in Pro-Mix BX Mycorrhizae potting mix. The seeds were planted 1.5 cm deep in 22 cm tall Cone-tainer pots with a 3.8 cm diameter (Ray Leach SC10R, Stuewe & Sons, Inc.). Plants were first grown in a PGC-105 growth chamber (Percival Scientific, Perry, IA) with a ~450 μmol·m−2·s−1 day period of 16 h at 25°C and a dark night period of 8 h at 17°C and watered daily.Sequencing - Sequencing performed by Plasmidsaurus, please see accompanying manuscript for more detail.Sample Collection - At the onset of peduncle elongation, plants were transferred from long-days conditions with photocycles and thermocycles to the imaging chamber under constant light with thermocycles. The CESA8 bright zone of the peduncle was harvested at seven time points over the course of one day, with four biological replicates per time point. Each replicate comprised three segments collected from the tallest stem of three individual plants. Sampling began near the end of the warm daytime period, 30 min prior to the transition to nighttime temperature (ZT15.5), followed by three time points during the cooler night (ZT16.5, ZT20, and ZT23.5), and three time points after the onset of the warm daytime period, which began at ZT24 (ZT24.5, ZT27, ZT32).Library Construction - RNA quality assessment, library preparation, and sequencing were performed by Plasmidsaurus using an Illumina platform with a 3’ end counting approach.Nucleic Acid Extraction - Total RNA was extracted from each time point using the Plant RNeasy mini kit (Qiagen) according to manufacturer instructions, including on-column treatment digestions with RNase-free DNase I.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesBahman KhahanifalseRNA-seq analysis of CESA8pro:LUC under different time-pointsTo better resolve the pronounced transcriptional changes observed around temperature transitions, we increased sampling density at the boundaries of the thermocycle.2026-05-20T00:00:00Z2026-05-20T20:28:05.773Z2026-01-12T16:06:44.334ZE-MTAB-16515ERP187487EFO_0002944EFO_0004170EFO_0003789EFO_0005518EFO_0003738EFO_0004184