{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Mallikarjuna rao Kovi"],"instrument_platform":["Illumina NovaSeq X"],"study_type":["genotyping by high throughput sequencing"],"organism":["Phleum pratense"],"species":["Phleum pratense"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16539"],"description":["The experiment was aimed at studying allele frequency changes and loci under selection in three temporal populations of two timothy cultivars; Engmo (Early=1988, Mid=2003 & Current=2020) and Noreng (Early=1998, Mid=2010 & Current=2020)."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Growth Protocol - Plants were grown for 8 weeks at ambient room temperature in green houses","Sequencing - Specific-Locus Amplified Fragment Sequencing (SLAF-Seq) by BMKgene","Sample Collection - For population genetic studies, approximately 80–100 seeds of each population were sown in two pots and cultivated at ambient room temperatures for 8 weeks. For DNA extraction, approximately an equal amount of tissue from 30 randomly selected plants from each pot was collected and flash-frozen in liquid nitrogen.","Library Construction - restriction enzymes - RsaI and HaeIII were choosen to uniformly cover the genome based on pre-experiment","Nucleic Acid Extraction - DNA extractions were carried out using Promega wizard HMW DNA extraction kit according to the manufacturer’s guidelines"],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Mallikarjuna rao Kovi"],"additional_accession":[]},"is_claimable":false,"name":"Allele frequency changs in temporal populations of two timothy cultivars","description":"The experiment was aimed at studying allele frequency changes and loci under selection in three temporal populations of two timothy cultivars; Engmo (Early=1988, Mid=2003 & Current=2020) and Noreng (Early=1998, Mid=2010 & Current=2020).","dates":{"release":"2026-03-09T00:00:00Z","modification":"2026-06-16T17:30:30.285Z","creation":"2026-01-16T18:52:43.377Z"},"accession":"E-MTAB-16539","cross_references":{"ENA":["ERP187671"],"Biostudies":["E-MTAB-14732"],"EFO":["EFO_0002944","EFO_0004170","EFO_0003789","EFO_0002771","EFO_0005518","EFO_0004184"]}}