biostudies-arrayexpressUnknownTranscriptomicsGenomicsProteomicsMallikarjuna rao KoviIllumina NovaSeq Xgenotyping by high throughput sequencingPhleum pratensePhleum pratensehttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16539The experiment was aimed at studying allele frequency changes and loci under selection in three temporal populations of two timothy cultivars; Engmo (Early=1988, Mid=2003 & Current=2020) and Noreng (Early=1998, Mid=2010 & Current=2020).biostudies-arrayexpressGrowth Protocol - Plants were grown for 8 weeks at ambient room temperature in green housesSequencing - Specific-Locus Amplified Fragment Sequencing (SLAF-Seq) by BMKgeneSample Collection - For population genetic studies, approximately 80–100 seeds of each population were sown in two pots and cultivated at ambient room temperatures for 8 weeks. For DNA extraction, approximately an equal amount of tissue from 30 randomly selected plants from each pot was collected and flash-frozen in liquid nitrogen.Library Construction - restriction enzymes - RsaI and HaeIII were choosen to uniformly cover the genome based on pre-experimentNucleic Acid Extraction - DNA extractions were carried out using Promega wizard HMW DNA extraction kit according to the manufacturer’s guidelinesOrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesMallikarjuna rao KovifalseAllele frequency changs in temporal populations of two timothy cultivarsThe experiment was aimed at studying allele frequency changes and loci under selection in three temporal populations of two timothy cultivars; Engmo (Early=1988, Mid=2003 & Current=2020) and Noreng (Early=1998, Mid=2010 & Current=2020).2026-03-09T00:00:00Z2026-03-09T02:02:43.136Z2026-01-16T18:52:43.377ZE-MTAB-16539ERP187671E-MTAB-14732EFO_0002944EFO_0004170EFO_0003789EFO_0002771EFO_0005518EFO_0004184