biostudies-arrayexpressMathys DelattreMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16570The autosomal-dominant Y2334N mutation in PRPF8 is associated with retinitis pigmentosa, a progressive retinal disorder. Here, we investigated how the Y2334N mutation affects circRNA differential expression in the retinas of homozygous Y2334N mutant mice.biostudies-arrayexpressNucleic Acid Extraction - Neural retina samples were resuspended in 300 µL of TRIzol and then stored at -80°C. The total RNA was isolated from each sample using the Direct-zol RNA MicroPrep Kit (Cat. No R2062) according to the manufactuerer's instructions. Then, each sample was on-column DNase I treated. Finally, RNA was eluted in 11 µL of DNase/RNase-free water.Library Construction - Ribodepletion and library preparation was done with KAPA RNA HyperPrep Kit with RiboErase KK8560. Then the quality and length distribution was mesured using Agilent 2100 Bioanalyser and Qubit. Pooling was done and primer-dimers were cleaned using Spri-selected beads. A final pool quality and length distribution was measured with Agilent 2100 Bioanalyser and Qubit.Sequencing - Libraries were sequenced on an Illumina NextSeq 2000 instrument using XLEAP-SBS chemistry with P3 flow cells, producing paired-end 150bp reads (300 cycles). Libraries were sequenced on an Illumina NextSeq 2000 platform using XLEAP-SBS chemistry with P3 flow cells, producing paired-end 150 bp reads.Sample Collection - The mice have GFP-tagged PRPF8 on both alleles with the Y2334N mutation for the mutant samples, and no mutation for the control samples. The neural retina was isolated from the mice and immediately frozen in dry ice.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesData Transformation - Sequencing reads were obtained with BCLconvert v4.3.13. The FASTQ files were then processed by CIRIquant v1.1.3 to quantify backsplice junctions (BSJ) and forward splice junction (FSJ), using the reference genome GRCm39 (Ensembl annotation version 112).MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000RNA-seq of coding RNAMus musculusFelix ZimmannDavid StanekMathys DelattrefalseAnalysis of differential circRNA expression in mouse retina isolated from mice that harbor the Y2334N mutation in both alleles of the pre-mRNA splicing factor PRPF8The autosomal-dominant Y2334N mutation in PRPF8 is associated with retinitis pigmentosa, a progressive retinal disorder. Here, we investigated how the Y2334N mutation affects circRNA differential expression in the retinas of homozygous Y2334N mutant mice.2026-02-19T00:00:00Z2026-05-30T17:05:19.787Z2026-01-26T11:45:34.668ZE-MTAB-16570ERP188134E-MTAB-15271EFO_0002944EFO_0004170EFO_0005518EFO_0003816EFO_0003738EFO_0004184