biostudies-arrayexpressMateusz JundzillStaphylococcus aureushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16578S. aureus isolates from chronic rhinosinusitis patients and healthy individuals were cultured overnight. The strains belonged to clonal complexes CC7 (CSS66 and MIN142) and CC15 (CSS126 and MIN93) that are both prevalent in Germany. These isolates have previously been characterized in Tuchscherr, L., et al., Reduced Glycolysis and Cytotoxicity in Staphylococcus aureus Isolates from Chronic Rhinosinusitis as Strategies for Host Adaptation. Int J Mol Sci, 2024. 25(4). Cultures were diluted in TSB broth to an optical density (OD600) of 0.05 the following day, and samples were collected at 3- and 6-hours post-dilution. Total RNA was extracted at these time-points and subjected to RNA sequencing (RNA-seq).biostudies-arrayexpressLibrary Construction - Nucleic acid library was prepared using standard Illumina RNA-seq protocol that uses random hexamer primers that for cDNA synthesis.Sequencing - RNA-seq was performed on Illumina NovaSeq PE 150 device 2 × 150 bp Paired-end reads sequencing.Nucleic Acid Extraction - RNA from harvested samples were isolated using Zymo Research Quick-RNA Fungal/Bacterial Miniprep Kit standard protocol and rRNA was depleted using Invitrogen MICROBExpress™ Bacterial mRNA Enrichment Kit kit.Sample Collection - S. aureus isolates from chronic rhinosinusitis patients (CSS) and healthy individuals (MIN) were cultured overnight.Cultures were diluted in TSB broth to an optical density (OD600) of 0.05 the following day, and samples were collected at 3- and 6-hours post-dilution.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesUnknownTranscriptomicsGenomicsProteomicsIllumina NovaSeq 6000RNA-seq of coding RNAStaphylococcus aureusTranscriptional Reprogramming of Staphylococcus aureus in Chronic Rhinosinusitis Reveals a Persistence-associated Phenotype by RNA SequencingMateusz JundzillLorena TuchscherrLorena Tuchscherr, Stefan Monecke, Mateusz Jundzill, Martin Hölzer, Christian Brandt, Sindy Wendler, Juliane Priese, Ralf Ehricht and Orlando Guntinas-LichiusfalseRNA-seq of Staphylococcus aureus isolates from chronic rhinosinusitis patients (CSS) against healthy individualsS. aureus isolates from chronic rhinosinusitis patients and healthy individuals were cultured overnight. The strains belonged to clonal complexes CC7 (CSS66 and MIN142) and CC15 (CSS126 and MIN93) that are both prevalent in Germany. These isolates have previously been characterized in Tuchscherr, L., et al., Reduced Glycolysis and Cytotoxicity in Staphylococcus aureus Isolates from Chronic Rhinosinusitis as Strategies for Host Adaptation. Int J Mol Sci, 2024. 25(4). Cultures were diluted in TSB broth to an optical density (OD600) of 0.05 the following day, and samples were collected at 3- and 6-hours post-dilution. Total RNA was extracted at these time-points and subjected to RNA sequencing (RNA-seq).2026-02-12T00:00:00Z2026-02-12T02:01:03.09Z2026-01-27T14:42:54.387ZE-MTAB-16578ERP188215EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184