{"database":"biostudies-arrayexpress","file_versions":[],"scores":null,"additional":{"omics_type":["Metabolomics","Unknown","Transcriptomics","Genomics","Proteomics"],"submitter":["Data Submission IFOM - ETS"],"instrument_platform":["MinION"],"study_type":["DNA-seq"],"organism":["Homo sapiens"],"species":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/E-MTAB-16581"],"description":["Nanopore sequencing data of rolling-circle amplification (RCA) products performed on hES cells, treated with 2mJ UV-C to induce apoptosis. The experimental conditions were designed to characterize circular DNA forms generated during apoptosis. gDNA from untreated and UV-treated cells are used as controls."],"repository":["biostudies-arrayexpress"],"sample_protocol":["Sample Collection - Cells were collected by treatment with accutase, a gentle enzymatic dissociation reagent for detaching adherent cells.","Nucleic Acid Extraction - Total DNA was isolated using phenol-chloroform extraction followed by ethanol precipitation and resuspension in nuclease-free water.","Sequencing - Libraries were sequenced on the Oxford Nanopore MinION platform using standard flow cells with real-time basecalling","Library Construction - Libraries were prepared using the Oxford Nanopore Native Barcoding Kit for multiplexed long-read sequencing applications."],"figure_sub":["Organization","MINSEQE Score","Assays and Data","MAGE-TAB Files"],"pubmed_authors":["Ylli Doksani","Elia Zanella","Data Submission IFOM - ETS"],"additional_accession":[]},"is_claimable":false,"name":"Nanopore sequencing data of rolling-circle amplification products from apoptotic cells and its relative gDNA control","description":"Nanopore sequencing data of rolling-circle amplification (RCA) products performed on hES cells, treated with 2mJ UV-C to induce apoptosis. The experimental conditions were designed to characterize circular DNA forms generated during apoptosis. gDNA from untreated and UV-treated cells are used as controls.","dates":{"release":"2026-04-30T00:00:00Z","modification":"2026-04-30T01:01:53.644Z","creation":"2026-01-27T17:25:58.773Z"},"accession":"E-MTAB-16581","cross_references":{"ENA":["ERP188218"],"EFO":["EFO_0002944","EFO_0004170","EFO_0002693","EFO_0005518","EFO_0004184"]}}