biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsData Submission IFOM - ETSMinIONDNA-seqHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16581Nanopore sequencing data of rolling-circle amplification (RCA) products performed on hES cells, treated with 2mJ UV-C to induce apoptosis. The experimental conditions were designed to characterize circular DNA forms generated during apoptosis. gDNA from untreated and UV-treated cells are used as controls.biostudies-arrayexpressSample Collection - Cells were collected by treatment with accutase, a gentle enzymatic dissociation reagent for detaching adherent cells.Nucleic Acid Extraction - Total DNA was isolated using phenol-chloroform extraction followed by ethanol precipitation and resuspension in nuclease-free water.Sequencing - Libraries were sequenced on the Oxford Nanopore MinION platform using standard flow cells with real-time basecallingLibrary Construction - Libraries were prepared using the Oxford Nanopore Native Barcoding Kit for multiplexed long-read sequencing applications.OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesYlli DoksaniElia ZanellaData Submission IFOM - ETSfalseNanopore sequencing data of rolling-circle amplification products from apoptotic cells and its relative gDNA controlNanopore sequencing data of rolling-circle amplification (RCA) products performed on hES cells, treated with 2mJ UV-C to induce apoptosis. The experimental conditions were designed to characterize circular DNA forms generated during apoptosis. gDNA from untreated and UV-treated cells are used as controls.2026-04-30T00:00:00Z2026-04-30T01:01:53.644Z2026-01-27T17:25:58.773ZE-MTAB-16581ERP188218EFO_0002944EFO_0004170EFO_0002693EFO_0005518EFO_0004184