biostudies-arrayexpressJurjun VeldeHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16601Deep RNA sequencing was performed to investigate the transcriptomic effects of siRNA-mediated knockdown of SRSF7 or SMNDC1 in the triple-negative breast cancer cell line MDA-MB-231. A total of 30 million cells MDA-MB-231 cells were seeded in 6-well plates. The following day, cells were transfected with siKinasePool siRNAs targeting SRSF7, SMNDC1, or a mock control. Samples from three biological replicates were collected 72 hours after transfection. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Stranded PolyA-selected libraries were prepared and sequenced on the DNBseq platform, generating 150-bp paired-end reads on a DNBSEQ-G400 sequencer (BGI Europe).biostudies-arrayexpressNucleic Acid Extraction - Total RNA was isolated using the RNeasy plus mini kit (Qiagen). Samples from 3 biological replicates were used. RNA quality and quantity were measured on an Agilent-4200 Bioanalyzer.Library Construction - Stranded PolyA-selected libraries were prepared using the DNBseq platform.Sample Collection - A total of 30 million cells MDA-MB-231 cells were seeded in 6-well plates. The following day, cells were transfected with siKinasePool siRNAs targeting SRSF7, SMNDC1, or a mock control. Samples from three biological replicates were collected 72 hours after transfection.Sequencing - 100 M 150-bp paired-end reads were sequenced on a DNBSEQ-G400 sequencer by BGI Europe.OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Reads were aligned against the human GRCH38 reference genome (gencode version 45) using the STAR aligner (version 2.7.11b).Data Transformation - Gene expression quantification was performed concurrently during mapping with the STAR --quantMode option set to GeneCounts.UnknownTranscriptomicsGenomicsProteomicsDNBSEQ-G400RNA-seq of coding RNAHomo sapiensBob WaterSylvia DévédecJurjun VeldefalseRNA seq of human breast cancer cell line MDAMB231 with knockdown of splicing factors SRSF7 and SMNDC1Deep RNA sequencing was performed to investigate the transcriptomic effects of siRNA-mediated knockdown of SRSF7 or SMNDC1 in the triple-negative breast cancer cell line MDA-MB-231. A total of 30 million cells MDA-MB-231 cells were seeded in 6-well plates. The following day, cells were transfected with siKinasePool siRNAs targeting SRSF7, SMNDC1, or a mock control. Samples from three biological replicates were collected 72 hours after transfection. Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Stranded PolyA-selected libraries were prepared and sequenced on the DNBseq platform, generating 150-bp paired-end reads on a DNBSEQ-G400 sequencer (BGI Europe).2026-06-01T00:00:00Z2026-06-01T01:01:02.085Z2026-01-29T11:22:13.327ZE-MTAB-16601ERP188295EFO_0002944EFO_0004170EFO_0004917EFO_0005518EFO_0003816EFO_0003738EFO_0004184