biostudies-arrayexpressAdam Yongxin YeMus musculushttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16602We performed ATAC-seq in v-Abl transformed pro-B cells and its various mutant derivatives to study the differential molecular mechanisms of Igk secondary V(D)J recombination.biostudies-arrayexpressSample Treatment - G1-arrest was performed by treating v-Abl cell lines with 3uM STI for 4 daysLibrary Construction - Libraries were PCR-amplified with Illumina primers and subjected to sequencing.Growth Protocol - v-Abl cell lines were cultured in RPMI1640 media with 15% FBSSample Collection - 50,000 G1-arrested RAG-deficient v-Abl cells were used for experimentNucleic Acid Extraction - Briefly, the collected G1-arrest live cells were used for cell lysis, nuclei preparation, tagmentation by Tn5 and library amplification according to Zymo-Seq ATAC Library Kit (D5458).Sequencing - ATAC-Seq Final ATAC-Seq libraries were sequenced on Illumina NextSeq2000OrganizationMINSEQE ScoreAssays and DataProcessed DataMAGE-TAB FilesSequence Alignment - Reads were processed using Nextflow ATAC-seq pipeline (https://github.com/nf-core/atacseq)Data Transformation - All bigWig files were scaling normalized to 1 million mapped reads.MetabolomicsUnknownTranscriptomicsGenomicsProteomicsNextSeq 2000ATAC-seqMus musculusAdam Yongxin YeFrederick AltfalseATAC-seq for Linear RAG Scanning Mediates Editing of Igκ Variable Region RepertoiresWe performed ATAC-seq in v-Abl transformed pro-B cells and its various mutant derivatives to study the differential molecular mechanisms of Igk secondary V(D)J recombination.2026-02-23T00:00:00Z2026-05-27T13:42:23.508Z2026-01-29T13:07:18.971ZE-MTAB-16602ERP188307E-MTAB-16001E-MTAB-116007E-MTAB-16014EFO_0002944EFO_0007045EFO_0004170EFO_0003789EFO_0004917EFO_0005518EFO_0003816EFO_0004184EFO_0003969