biostudies-arrayexpressMetabolomicsUnknownTranscriptomicsGenomicsProteomicsData Submission IFOM - ETSMinIONRNA-seq of coding RNAHomo sapiensHomo sapienshttps://www.ebi.ac.uk/biostudies/studies/E-MTAB-16605We performed direct cDNA sequencing in HeLa GFP∆Promoter cells by Oxford Nanopore Technology (ONT) on a MinION device to detect EGFP RNA levels after DSB induction.biostudies-arrayexpressLibrary Construction - RNA from each sample was depleted of the ribosomal RNA using the Illumina TruSeq Stranded Total RNA Library Prep Gold and used for library preparation. The complementary DNA strand was synthesized using an oligo dT adapter supplied by the kit. Then, adaptor-motor protein complexes were ligated to cDNA ends to allow cDNA to enter the pores for sequencing.Sequencing - Libraries were loaded on R9 flow cells using the Flow Cell Priming Kit (EXP-FLP002) and sequenced individually on MinION 1X device. Only one strand of the duplex is sequenced, producing 1D reads.Sample Collection - Cells were collected and washed with PBS 1x and RNA was extractedNucleic Acid Extraction - The cytoplasmic RNA fraction from HeLa GDP cells, infected with either scr-C9 or sg-C9, was extracted using Maxwell RSC simplyRNA Tissue Kit (Promega).OrganizationMINSEQE ScoreAssays and DataMAGE-TAB FilesData Submission IFOM - ETSSara TavellaFabrizio D'Adda Di FagagnafalseDetecting EGFP RNA transcripts by Oxford Nanopore TechnologyWe performed direct cDNA sequencing in HeLa GFP∆Promoter cells by Oxford Nanopore Technology (ONT) on a MinION device to detect EGFP RNA levels after DSB induction.2026-05-15T00:00:00Z2026-05-15T01:00:41.439Z2026-02-09T13:34:40.217ZE-MTAB-16605ERP188890EFO_0002944EFO_0004170EFO_0005518EFO_0003738EFO_0004184